Cell Dissociation Buffer, enzyme-free, PBS

Cell Dissociation Buffer, enzyme-free, PBS - FAQs

View additional product information for Cell Dissociation Buffer, enzyme-free, PBS - FAQs (13151014)

5 product FAQs found

What is the osmolality and pH range for Cell Dissociation Buffer, enzyme-free, PBS (Cat. No. 13151014)

Below are QC test specifications for pH and osmolality for this product:

- pH: 6.9 to 7.9
- Osmolality: 289 to 321 mOsm/kg

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

At what confluency should cells be at prior to their dissociation using the Cell Association Buffer, enzyme-free, PBS?

The buffer should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, it will be hard to detach them.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the Cell Dissociation Buffer, enzyme-free, PBS damage cell surface markers/receptors?

This buffer is very gentle. It should preserve the structural and functional integrity of cell surface proteins. However, we recommend that the customer test their cells and proteins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use the Cell Dissociation Buffer, enzyme-free, PBS to dissociate cells?

This Enzyme-Free Cell Dissociation Buffer does not rely on proteolysis for its action. Therefore, it is not suitable for strongly adherent cells, or for routine passage of some cell types (e.g., A431, Hep2, A549, MDCK, WI-38). This product is intended to gently dissociate cells from attachment substrates and each other while maintaining surface protein integrity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.