Invitrogen

Ubiquitin Monoclonal Antibody (Ubi-1)

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49 Published Figures
118 References
View all (59) Ubiquitin antibodies

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Product Details

13-1600

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
View 79 publications 79 publications

Immunohistochemistry (IHC)

Assay-dependent
View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 9 publications 9 publications

ELISA (ELISA)

0.1-1 µg/mL
View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent
View 8 publications 8 publications

Miscellaneous PubMed (Misc)

-
View 5 publications 5 publications
Product Specifications

Species Reactivity

All, Bovine, Chicken, Human, Mouse, Xenopus

Published species

Avian, C. elegans, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat, Rhesus monkey, Tag, Xenopus, Yeast

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Ubi-1

Immunogen

Purified bovine ubiquitin conjugated to carrier protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533002

Product Specific Information

13-1600 recognizes ubiquitin, both conjugated and unconjugated. It reacts with a single chain 8.5 kDa protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Ubiquitin is present in the nucleus, cytoplasm, and on cell surface membranes.

This antibody is suitable for immunohistochemical staining of alcohol- or paraformaldehyde-fixed, paraffin-embedded or frozen tissue sections. Heat-induced epitope retrieval with citrate buffer, pH 6.0, is required for specific staining of formalin-fixed, paraffin-embedded tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C. This antibody may also be used in ELISA and has been successfully used in western blot analysis of ubiquitinated proteins in mouse thymocytes.

To perform western blotting, prepare lysis buffer in 10 mM N-Ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-Ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6 M guanidine-HCl, 20 mM Tris-HCl, pH 7.5, 5 mM betamercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4°C, followed by extensive PBS washing.

Target Information

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin; polyubiquitin B; polyubiquitin C; Polyubiquitin-B; Polyubiquitin-C; Ribosomal protein eS27-like; ribosomal protein S27-like; ribosomal protein S27-like protein; ribosomal protein S27a L homeolog; RPL40; RPS27A; RPS27L; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein L40; ubiquitin and ribosomal protein S27a; ubiquitin B; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin polyprotein (heat shock related); ubiquitin-40S ribosomal protein S27a; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein; ubiquitin-ribosomal protein fusion protein; ubiquitin/60S ribosomal fusion protein; ubiquitin/ribosomal protein; unnamed protein product

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Gene Aliases: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; AI194771; AL033289; CEP52; CEP80; D8Ertd21e; Gm1863; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; polyubiquitin-C; RPL40; RPS27A; rps27a.L; RPS27L; S27A; TI-225; UBA52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP2; UBCEP80; XELAEV_18028796mg

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UniProt ID: (Chicken) P0CG62, (Bovine) P62992, (Human) P62979, (Chicken) P79781, (Bovine) Q3T0B7, (Human) Q71UM5, (Mouse) P62983, (Mouse) Q6ZWY3, (Human) P62987, (Bovine) P63048, (Bovine) P0CG53, (Human) P0CG47, (Human) P0CG48, (Bovine) P0CH28, (Mouse) P62984, (Mouse) P0CG49, (Mouse) P0CG50

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Entrez Gene ID: (Chicken) 101747587, (Bovine) 286839, (Human) 6233, (Chicken) 395796, (Bovine) 614220, (Chicken) 415372, (Human) 51065, (Mouse) 78294, (Mouse) 67941, (Human) 7311, (Chicken) 395958, (Bovine) 615199, (Bovine) 281370, (Chicken) 396190, (Human) 7314, (Human) 7316, (Bovine) 444874, (Mouse) 22186, (Mouse) 22187, (Mouse) 22190, (Xenopus) 444494

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Function(s)
structural constituent of ribosome metal ion binding protein binding poly(A) RNA binding protein tag ubiquitin protein ligase binding translation activator activity cysteine-type endopeptidase activator activity involved in apoptotic process protease binding ribosomal protein translational protein
Process(es)
translation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade nuclear-transcribed mRNA catabolic process, nonsense-mediated decay activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling rRNA processing translational initiation SRP-dependent cotranslational protein targeting to membrane DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly viral transcription translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator DNA repair protein ubiquitination modification-dependent protein catabolic process activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator mitotic G1 DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of translation ribosomal small subunit assembly cellular response to DNA damage stimulus biological_process positive regulation of defense response to virus by host cellular protein modification process xenophagy male meiosis I female meiosis I female gonad development hypothalamus gonadotrophin-releasing hormone neuron development mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential fat pad development regulation of proteasomal protein catabolic process seminiferous tubule development energy homeostasis regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein monoubiquitination positive regulation of protein ubiquitination ubiquitin homeostasis male gonad development adipose tissue development
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