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Invitrogen
STAT5 alpha Monoclonal Antibody (ST5a-2H2)
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FIGURE: 1 / 20
STAT5 alpha Antibody (13-3600) in ICC/IF
Immunofluorescent analysis of STAT5 alpha was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT5 alpha Mouse Monoclonal Antibody (13-3600) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room tempera... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-3600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Tag,
Virus
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ST5a-2H2
Immunogen
A peptide corresponding to the C-terminus of murine Stat5a coupled to a protein carrier.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes the STAT5a protein from a variety of species including human, mouse, and rat and it reacts specifically with the STAT5a protein and does not exhibit any cross-reactivity with the highly related STAT5b protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Reactivity has been confirmed by western blotting using cell lysates derived from human A431 cells (+/- EGF treatment), NIH 3T3 cells, MCF-7 cells, and HeLa cells.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; lacks exon 18; mammary gland factor; mammary gland factor STAT5A; signal transducer and activator of transcription 5; STAT; STAT5B; transcription factor; unnamed protein product
Gene Aliases: MGF; STAT5
UniProt ID: (Human) P42229, (Rat) Q62771, (Mouse) P42230
Entrez Gene ID: (Human) 6776, (Rat) 24918, (Mouse) 20850
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
phosphate ion binding
sequence-specific DNA binding
DNA-binding transcription factor binding
promoter-specific chromatin binding
double-stranded DNA binding
luteinization
natural killer cell differentiation
positive regulation of endothelial cell proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
defense response
signal transduction
JAK-STAT cascade
female pregnancy
lactation
positive regulation of cell proliferation
gene expression
peptidyl-tyrosine phosphorylation
regulation of steroid metabolic process
cytokine-mediated signaling pathway
taurine metabolic process
lipid storage
regulation of cell adhesion
eosinophil differentiation
regulation of epithelial cell differentiation
mammary gland development
response to nutrient levels
positive regulation of interleukin-2 production
positive regulation of natural killer cell differentiation
negative regulation of mast cell apoptotic process
T cell differentiation in thymus
interleukin-15-mediated signaling pathway
interleukin-4-mediated signaling pathway
reelin-mediated signaling pathway
interleukin-5-mediated signaling pathway
interleukin-2-mediated signaling pathway
interleukin-7-mediated signaling pathway
interleukin-9-mediated signaling pathway
interleukin-3-mediated signaling pathway
thrombopoietin-mediated signaling pathway
regulation of multicellular organism growth
positive regulation of multicellular organism growth
positive regulation of activated T cell proliferation
regulation of cell proliferation
T cell homeostasis
negative regulation of apoptotic process
response to peptide hormone
positive regulation of blood vessel endothelial cell migration
response to ethanol
positive regulation of B cell differentiation
positive regulation of gamma-delta T cell differentiation
positive regulation of lymphocyte differentiation
negative regulation of erythrocyte differentiation
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
positive regulation of natural killer cell mediated cytotoxicity
development of secondary female sexual characteristics
development of secondary male sexual characteristics
Peyer's patch development
gland development
positive regulation of inflammatory response
mammary gland involution
positive regulation of mast cell differentiation
JAK-STAT cascade involved in growth hormone signaling pathway
prostate gland epithelium morphogenesis
epithelial cell differentiation involved in prostate gland development
mammary gland epithelium development
positive regulation of mast cell proliferation
cellular response to cytokine stimulus
cellular response to granulocyte macrophage colony-stimulating factor stimulus
negative regulation of T-helper 17 cell lineage commitment
regulation of hemopoiesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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