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Invitrogen
Perlecan Monoclonal Antibody (7B5)
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FIGURE: 1 / 15
Perlecan Antibody (13-4400) in ICC/IF
Immunofluorescence analysis of HSPG2 was performed using 70% confluent log phase HUVEC and SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Perlecan Monoclonal Antibody (7B5) (Product # 13-4400, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Produ... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20220826?time=20220826 "Perlecan Antibody (13-4400) in IHC")
.jpg?time=20220826?time=20220826 "Perlecan Antibody (13-4400) in WB")
Product Details
13-4400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7B5
Immunogen
rec-Perlecan domain III.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: endorepellin (domain V region); perlecan proteoglycan; putative proteoglycan; matrix protein; RP11-132G19.2
Gene Aliases: HSPG; PLC; PRCAN; SJA; SJS; SJS1
UniProt ID: (Human) P98160
Entrez Gene ID: (Human) 3339
lipid metabolic process
receptor-mediated endocytosis
inflammatory response
brain development
negative regulation of angiogenesis
cell differentiation
negative regulation of synaptic transmission, cholinergic
protein localization to synapse
negative regulation of neuron apoptotic process
circulatory system development
negative regulation of amyloid fibril formation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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