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Invitrogen
Tau Monoclonal Antibody (T46)
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FIGURE: 1 / 42
Tau Antibody (13-6400) in ICC/IF
Immunofluorescent analysis of Tau was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Tau Mouse Monoclonal Antibody (Product # 13-6400) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-6400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Human,
Mouse,
Rabbit,
Rat,
Xenopus
Published species
Fruit fly,
Human,
Mouse,
Non-human primate,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
T46
Immunogen
Bovine Tau.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an epitope near the C-terminal region (404-441 aa) of human tau. It reacts with all 7 isoforms of human tau (binding-competent and binding-incompetent), A68 (an abnormal phosphorylated derivative of tau), bovine tau, and human NeuroFibrillary Tangles (NFTs).
Reactivity is independent of the state of tau phosphorylation. Some cross-reactivity with MAP2 is also observed.
This antibody is suitable for immunohistochemical staining of Bouin's-fixed or alcohol-fixed, paraffin-embedded or frozen tissue sections.
Target Information
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ31424; FTDP17; map tau; MGC138549; Microtubule-associated protein tau; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; Tau microtubule-associated protein; Tau-4; Tau5
Gene Aliases: AI413597; AW045860; MAPT; Mtapt; pTau; RNPTAU; TAU
UniProt ID: (Bovine) P29172, (Mouse) P10637
Entrez Gene ID: (Bovine) 281296, (Chicken) 426737, (Rabbit) 100344003, (Rat) 29477, (Mouse) 17762
positive regulation of diacylglycerol kinase activity
microtubule cytoskeleton organization
neuron migration
apoptotic process
microtubule-based process
axonogenesis
brain development
female pregnancy
response to nutrient
adult walking behavior
axo-dendritic transport
intrinsic apoptotic signaling pathway in response to oxidative stress
response to organic substance
regulation of autophagy
neuron differentiation
positive regulation of microtubule polymerization
negative regulation of intracellular transport
positive regulation of axon extension
mitochondrion transport along microtubule
axon extension
regulation of microtubule-based movement
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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