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Invitrogen
Phosphotyrosine Antibody Cocktail, HRP
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FIGURE: 1 / 1
Phosphotyrosine Antibody (13-6620) in WB
Cell death & disease 2021 -
Fig. 1 Phosphorylation of HDAC1 at Tyr72 is critical for the expression of HDAC1 protein. a Immunoprecipitation of endogenous HDAC1 and Western blot analysis to detect tyrosine phosphorylation in A549 and PC-9 cells with the indicated antibodies. b Predicted tyrosine phosphorylation sites in HDAC1 detected in non-small cell lung cancer cells. c Predicted tyrosine phosphorylation sites were mutated to the no... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-6620
Applications
Tested Dilution
Publications
Product Specifications
Published species
Chemical,
Human
Host/Isotype
Mouse
/ IgG1/IgG2b
Class
Cocktail
Type
Antibody
Clone
PY-7E1, PY20
Immunogen
Tyrosine-phosphorylated proteins.
Conjugate
HRP
HRP
HRP
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
13-6200 contains a cocktail of monoclonal mouse Phosphotyrosine containing clone PY-7E1, isotype IgG1, kappa and clone PY20, isotype IgG2b.
Target Information
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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