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Invitrogen
Connexin 43 Monoclonal Antibody (CX-1B1)
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FIGURE: 1 / 8
Connexin 43 Antibody (13-8300) in IHC (P)
Immunohistochemistry analysis of Connexin 43 showing staining in the membrane of paraffin-embedded human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temp... View More
Product Details
13-8300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Bat,
Cat,
Dog,
Hamster,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Rodent
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CX-1B1
Immunogen
Synthetic peptide corresponding to a cytoplasmic sequence located near the C-terminus of rat Connexin 43.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
CX-1B1 is specific for one of the unphosphorylated forms of Connexin 43. This antibody was at one time thought to be specific for all unphosphorlyated forms of Connexin 43, but that can no longer be claimed since it was discovered that Connexin 43 can be phosphorylated at multiple sites. Recent studies have shown that phosphorylation occurs at a serine within the epitope recognized by this antibody. CX-1B1 recognizes Connexin 43 only when the serine at residue 368 is unphosphorylated. CX-1B1 will, however, recognize Connexin 43 if it is phosphorylated at sites besides Ser368. In some cell types, cross-reactivity with a ~70 kDa protein of unknown identity has been observed. This cross-reactivity is likely due to a shared epitope.
Reactivity has been confirmed by western blot analysis of extracts derived from dog heart, human heart, mouse brain, mouse heart, rat brain, and Clone 9 (C1-9) rat liver cells. Clone 9 is the recommended positive control for CX-1B1.
This antibody has also been used in immunofluorescence applications and immunolocalization of Connexin 43, however, for immunohistochemical applications polyclonal Rabbit anti-Connexin 43 (Cat. No. 71-0700) is recommended.
Target Information
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); GJA1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product
Gene Aliases: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; EKVP3; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA
UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050
Entrez Gene ID: (Human) 2697, (Dog) 403418, (Mouse) 14609, (Rat) 24392
gap junction channel activity
protein binding
beta-catenin binding
ion transmembrane transporter activity
efflux transmembrane transporter activity
tubulin binding
transmembrane transporter activity
glutathione transmembrane transporter activity
alpha-tubulin binding
gap junction hemi-channel activity
gap junction channel activity involved in cardiac conduction electrical coupling
gap junction channel activity involved in cell communication by electrical coupling
signal transducer activity
receptor binding
SH3 domain binding
protein domain specific binding
PDZ domain binding
beta-tubulin binding
connexin binding
scaffold protein binding
gap junction
establishment of mitotic spindle orientation
cardiac conduction system development
cell communication
signal transduction
cell-cell signaling
spermatogenesis
heart development
protein localization
positive regulation of gene expression
cell communication by electrical coupling
glutamate secretion
gap junction assembly
negative regulation of cell growth
negative regulation of gonadotropin secretion
ion transmembrane transport
glutathione transmembrane transport
maintenance of blood-brain barrier
xenobiotic transport
positive regulation of I-kappaB kinase/NF-kappaB signaling
cell-cell junction organization
bone remodeling
transmembrane transport
bone development
atrial cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
microtubule-based transport
positive regulation of cold-induced thermogenesis
export across plasma membrane
negative regulation of trophoblast cell migration
regulation of blood circulation
cellular response to beta-amyloid
positive regulation of vascular smooth muscle cell proliferation
positive regulation of morphogenesis of an epithelium
positive regulation of mesodermal cell differentiation
positive regulation of stem cell proliferation
regulation of apoptotic process
osteoblast differentiation
in utero embryonic development
neuron migration
negative regulation of endothelial cell proliferation
heart looping
regulation of heart rate
epithelial cell maturation
lens development in camera-type eye
chronic inflammatory response
positive regulation of glomerular filtration
atrial ventricular junction remodeling
epicardial cell to mesenchymal cell transition
apoptotic process
positive regulation of cytosolic calcium ion concentration
adult heart development
regulation of heart contraction
negative regulation of cell proliferation
response to pH
vascular transport
negative regulation of gene expression
cell communication by chemical coupling
positive regulation of cell communication by chemical coupling
ATP transport
regulation of bone mineralization
positive regulation of insulin secretion
embryonic heart tube development
embryonic digit morphogenesis
skeletal muscle tissue regeneration
positive regulation of osteoblast differentiation
positive regulation of protein catabolic process
positive regulation of striated muscle tissue development
positive regulation of vasoconstriction
positive regulation of vasodilation
regulation of bone remodeling
blood vessel morphogenesis
neuron projection morphogenesis
protein oligomerization
regulation of calcium ion transport
negative regulation of cardiac muscle cell proliferation
milk ejection
regulation of ventricular cardiac muscle cell membrane repolarization
regulation of blood vessel remodeling
regulation of atrial cardiac muscle cell membrane depolarization
regulation of ventricular cardiac muscle cell membrane depolarization
negative regulation of wound healing
cardiac conduction
establishment of epithelial cell polarity
negative regulation of DNA biosynthetic process
regulation of bicellular tight junction assembly
positive regulation of behavioral fear response
endothelium development
response to glucose
response to fluid shear stress
response to peptide hormone
cellular response to mechanical stimulus
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