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Invitrogen
ERK1 Monoclonal Antibody (ERK-6B11)
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FIGURE: 1 / 7
ERK1 Antibody (13-8600) in ICC/IF
Immunofluorescence analysis of ERK1 was performed using 70% confluent A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK1 (ERK-6B11) Mouse Monoclonal Antibody (Product # 13-8600) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-8600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Pig,
Rat,
Tag
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ERK-6B11
Immunogen
Synthetic Peptide corresponding to a section of the C-terminus of rat ERK1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ERK; Erk1; cancer susceptibility protein; ERK1; MAP kinase; mitogen-activated protein kinase; ERK1b; ERK1c; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase2; MAPK 1; MAPK 3; MAPK2; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; p44 MAP kinase; pp42/MAP kinase; protein serine/threonine kinase ERK1b; unnamed protein product
Gene Aliases: ERK-1; ERK1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MNK1; Mtap2k; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
UniProt ID: (Human) P27361, (Mouse) Q63844, (Rat) P21708
Entrez Gene ID: (Dog) 100855877, (Human) 5595, (Mouse) 26417, (Rat) 50689
phosphotyrosine binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
phosphatase binding
identical protein binding
protein serine kinase activity
DNA-binding transcription factor binding
scaffold protein binding
non-receptor serine/threonine protein kinase
MAPK cascade
negative regulation of T cell mediated immune response to tumor cell
protein phosphorylation
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
insulin receptor signaling pathway
positive regulation of macrophage chemotaxis
Schwann cell development
phosphorylation
sensory perception of pain
BMP signaling pathway
positive regulation of cyclase activity
positive regulation of telomere maintenance
regulation of stress-activated MAPK cascade
cellular response to amino acid starvation
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
ERBB2-ERBB3 signaling pathway
myelination
signal transduction in response to DNA damage
positive regulation of transcription from RNA polymerase II promoter
insulin-like growth factor receptor signaling pathway
modulation of synaptic transmission
stress-activated MAPK cascade
regulation of cytoskeleton organization
interleukin-34-mediated signaling pathway
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
interleukin-1-mediated signaling pathway
response to epidermal growth factor
cellular response to mechanical stimulus
caveolin-mediated endocytosis
regulation of Golgi inheritance
negative regulation of cholesterol efflux
positive regulation of macrophage proliferation
positive regulation of neuroinflammatory response
negative regulation of TORC1 signaling
regulation of early endosome to late endosome transport
autophagosome assembly
cellular response to DNA damage stimulus
signal transduction
response to toxic substance
organ morphogenesis
negative regulation of autophagy
positive regulation of autophagy
neural crest cell development
peptidyl-serine phosphorylation
regulation of ossification
thyroid gland development
lipopolysaccharide-mediated signaling pathway
cellular response to nutrient levels
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
response to lipopolysaccharide
TORC1 signaling
outer ear morphogenesis
response to exogenous dsRNA
positive regulation of translation
decidualization
thymus development
protein stabilization
negative regulation of T cell activation
cartilage development
regulation of intracellular pH
Bergmann glial cell differentiation
face development
lung morphogenesis
trachea formation
cardiac neural crest cell development involved in heart development
protein-containing complex assembly
cellular response to tumor necrosis factor
cellular response to toxic substance
positive regulation of xenophagy
arachidonic acid metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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