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Invitrogen

Connexin 43 Monoclonal Antibody (Connexin43 (1A)), eBioscience™

Advanced Verification
3 Published Figures
3 References
View all (42) Connexin 43 antibodies

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Product Details

14-4759-37

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL
-

Immunocytochemistry (ICC/IF)

Assay-Dependent
View 1 publication 1 publication

Flow Cytometry (Flow)

(0.5 µg)/test)
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Connexin43 (1A)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

2 mg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865077

Product Specific Information

Description: The Connexin43 monoclonal antibody recognizes mouse Connexin 43. Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication. Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins. Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties. Connexin 43 is expressed in many tissues, including the central nervous system, heart, and bone. Connexin 43 is the most abundant connexin found in the myocardium and is also the primary connexin expressed in astrocytes. Mutations in connexin 43 are associated with ocluodentodigital dysplasia, a rare developmental disorder characterized by craniofacial and limb abnormalities. Connexin 43 may also be a promising therapeutic target in the treatment of malignant melanomas and mammary gland tumors.

The monoclonal antibody Connexin43 also recognizes human and rat connexin 43.

Applications Reported: This Connexin43 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This Connexin43 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Connexin 43 (Cx43), encoded by the GJA1 gene, is a widely expressed gap junction protein that plays an important role in direct cell-to-cell communication. Six Cx43 proteins assemble to form a connexon, or hemichannel, which can pair with a connexon on a neighboring cell to create a gap junction channel. These channels allow ions, metabolites, and small signaling molecules to pass between adjacent cells, helping coordinate processes such as electrical conduction, tissue development, wound healing, and cellular homeostasis. Cx43 is especially abundant in tissues such as the heart, brain, bone, and skin, and changes in its expression, localization, or function have been associated with cardiovascular disease, neurological disorders, impaired tissue repair, and cancer. Because of its broad role in intercellular signaling and disease biology, Cx43 is an important target for studying gap junction function and developing potential therapeutic strategies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha 1 connexin; connexin 43; connexin k1; connexin-43; Cx-43; CXA1; DFNB38; gap junction 43 kDa heart protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; GJA1; Npm1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product; VEGF

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Gene Aliases: AVSD3; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxnk1; EKVP; EKVP3; Gja-1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

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UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Human) 2697, (Mouse) 14609, (Rat) 24392

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Function(s)
receptor binding gap junction channel activity protein binding beta-catenin binding ion transmembrane transporter activity efflux transmembrane transporter activity tubulin binding transmembrane transporter activity glutathione transmembrane transporter activity alpha-tubulin binding beta-tubulin binding gap junction hemi-channel activity gap junction channel activity involved in cardiac conduction electrical coupling scaffold protein binding gap junction channel activity involved in cell communication by electrical coupling protein tyrosine kinase binding channel activity SH3 domain binding protein domain specific binding PDZ domain binding connexin binding disordered domain specific binding
Process(es)
establishment of mitotic spindle orientation osteoblast differentiation in utero embryonic development neuron migration negative regulation of endothelial cell proliferation heart looping epithelial cell maturation lens development in camera-type eye chronic inflammatory response positive regulation of glomerular filtration atrial ventricular junction remodeling epicardial cell to mesenchymal cell transition cell communication signal transduction positive regulation of cytosolic calcium ion concentration cell-cell signaling spermatogenesis heart development adult heart development regulation of heart contraction protein localization negative regulation of cell proliferation male gonad development response to pH vascular transport positive regulation of gene expression negative regulation of gene expression cell communication by chemical coupling cell communication by electrical coupling regulation of cell communication by electrical coupling positive regulation of cell communication by chemical coupling glutamate secretion ATP transport negative regulation of cell growth regulation of bone mineralization positive regulation of insulin secretion negative regulation of gonadotropin secretion ion transmembrane transport glutathione transmembrane transport embryonic heart tube development maintenance of protein localization in endoplasmic reticulum T cell proliferation T cell activation vasodilation embryonic digit morphogenesis xenobiotic transport regulation of apoptotic process skeletal muscle tissue regeneration cell-cell junction organization positive regulation of protein catabolic process positive regulation of meiotic nuclear division positive regulation of striated muscle tissue development positive regulation of vasoconstriction bone remodeling regulation of bone remodeling blood vessel morphogenesis neuron projection morphogenesis regulation of body fluid levels regulation of calcium ion transport transmembrane transport negative regulation of cardiac muscle cell proliferation milk ejection regulation of ventricular cardiac muscle cell membrane repolarization regulation of blood vessel remodeling bone development regulation of atrial cardiac muscle cell membrane depolarization regulation of ventricular cardiac muscle cell membrane depolarization cardiac conduction cellular response to pH cell communication by electrical coupling involved in cardiac conduction establishment of epithelial cell polarity microtubule-based transport regulation of actin filament organization positive regulation of cold-induced thermogenesis export across plasma membrane negative regulation of trophoblast cell migration regulation of blood circulation positive regulation of establishment of Sertoli cell barrier cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation positive regulation of morphogenesis of an epithelium positive regulation of mesodermal cell differentiation negative regulation of DNA biosynthetic process positive regulation of stem cell proliferation regulation of bicellular tight junction assembly positive regulation of behavioral fear response response to ischemia endothelium development response to wounding response to glucose gap junction assembly response to estradiol response to lipopolysaccharide response to retinoic acid response to fluid shear stress cellular response to platelet-derived growth factor stimulus response to peptide hormone decidualization cellular response to mechanical stimulus cellular response to parathyroid hormone stimulus positive regulation of smooth muscle cell chemotaxis epididymis development cardiac conduction system development maintenance of blood-brain barrier positive regulation of I-kappaB kinase/NF-kappaB signaling atrial cardiac muscle cell action potential
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