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FIGURE: 1 / 56
CD4 Antibody (14-9766-37) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded mouse spleen using 5 µg/mL of Rat IgG1 K Isotype Control Purified (left) or 5 µg/mL of Anti-Mouse CD4 Purified (right) followed by Anti-Rat IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-9766-37
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4SM95
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
2 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 4SM95 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail.
The 4SM95 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD4 using immunohistochemistry of frozen tissue please refer to alternate products Anti-Mouse CD4 Purified (Product # 14-0041 and 14-0042).
Applications Reported: This 4SM95 antibody has been reported for use in western blotting, microscopy, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This 4SM95 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This 4SM95 antibody has also been tested by western blot of reduced mouse spleen lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD4 antigen p55; cd4a; fCD4; L3T4 differentiation antigen protein; L3T4 surface antigen precursor; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; unnamed protein product
Gene Aliases: L3T4; Ly-4
UniProt ID: (Mouse) P06332
Entrez Gene ID: (Mouse) 12504
virus receptor activity
receptor binding
protein binding
zinc ion binding
lipid binding
coreceptor activity
immunoglobulin binding
enzyme binding
protein kinase binding
MHC class II protein complex binding
interleukin-16 binding
interleukin-16 receptor activity
MHC class II protein binding
identical protein binding
protein homodimerization activity
protein tyrosine kinase binding
immune response
cell adhesion
cell surface receptor signaling pathway
positive regulation of calcium ion transport into cytosol
T cell differentiation
macrophage differentiation
maintenance of protein location in cell
helper T cell enhancement of adaptive immune response
interleukin-15-mediated signaling pathway
positive regulation of T cell proliferation
T cell activation
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
T cell selection
positive regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of viral entry into host cell
viral entry into host cell
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
regulation of T cell activation
positive regulation of T cell activation
regulation of calcium ion transport
positive regulation of ERK1 and ERK2 cascade
cellular response to granulocyte macrophage colony-stimulating factor stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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