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Invitrogen™

T4 DNA Ligase (1 U/μL)

Catalog number: 15224025
Invitrogen™

T4 DNA Ligase (1 U/μL)

Catalog number: 15224025
Catalog Number
15224025
also known as 15224-025
Unit Size
500 units
Price (USD)
Availability
-
Quantity
Catalog Number
15224017
also known as 15224-017
Unit Size
100 units
Price (USD)
Availability
-
Quantity
Catalog Number
15224090
also known as 15224-090
Unit Size
4 x 500 units
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
15224025
also known as 15224-025
500 units
-
15224017
also known as 15224-017
100 units
-
15224090
also known as 15224-090
4 x 500 units
-
Product Overview
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Additional Information
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T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.

Applications
Cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

Source
Purified from E. coli lambda lysogen NM989

Performance and quality testing
Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested

Unit definition
One unit catalyzes the exchange of 1 nmol 32P-labeled pyrophosphate into ATP in 20 min at 37°C. One unit is equal to approximately 300 cohesive-end ligation units.

Unit reaction conditions
66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, 3.3 μM 32P-labeled pyrophosphate, and enzyme in 0.1 mL for 20 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
1 U/μL
Shipping Condition
Dry Ice
Enzyme
Ligase
Compatible Buffer
5X Reaction Buffer
Quantity
500 U
Product Type
T4 DNA Ligase

Contents & Storage

T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2 , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

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