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Pierce™ Glutathione Coated Plates, Black, 96-Well

Catalog number: 15340

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of these Glutathione Coated Plates:

Selective—specifically binds fusion proteins containing glutathione S-transferase
Capacity—binds approx. 10 ng GST protein per well
Versatile—ready-to-bind plate format is useful for protein interaction studies
Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
Ready to use—Pre-blocked with BSA and SuperBlock Blocking Buffer to reduce nonspecific binding

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well polystyrene microplates whose wells have been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These glutathione coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.

Related Products
Pierce™ Glutathione Coated Plates, Clear, 8-Well Strip
Pierce™ Glutathione Coated Plates, White, 96-Well
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Binding Property: Affinity, Standard Capacity
Color: Black
Detection Method: Fluorescent
Format: 96-well plate
Plate Blocking: SuperBlock™ Buffer
Plate Type: Detection Plate, ELISA, Immunoassay
Product Line: Pierce™
Product Size: 5 plates
Surface Coating: Glutathione

Contents & storage

Store at 4°C in unopened pouches. Once opened, store unused plates in a resealable bag with desiccant and store at 4°C.

Documents

Manuals & protocols