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Invitrogen
F(ab')2-Goat anti-Mouse IgM (Mu chain) Secondary Antibody, Functional Grade, eBioscience™
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FIGURE: 1 / 1
Mouse IgM (Mu chain) Secondary Antibody (16-5092-85) in Flow
Mouse splenocytes were unstimulated (left) or stimulated with 10 µg/mL of F (ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified (Product # 16-5092-85) for 48 hours (right) followed by staining with Anti-Mouse CD19 PE (Product # 12-0193-82) and CD86 APC (Product # 17-0862-82).Cells in the lymphocyte gate were used for analysis.
Product Details
16-5092-85
Applications
Tested Dilution
Flow Cytometry (Flow)
10 µg/mL
Functional Assay (Functional)
Assay-Dependent
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ Ig
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgM
Antibody Form
F(ab')2 Fragment
Product Specific Information
Description: This polyclonal F(ab')2 fragment recognizes mouse IgM. Reactivity to human and bovine has been removed. This polyclonal antibody can be used to crosslink and activate B lymphocytes.
Applications Reported: This polyclonal F(ab')2 Goat Anti-Mouse IgM has been reported for use in functional assays (such as B cell activation). Concentrations should be determined empirically for optimal performance in the assay of interest.
Applications Tested: F(ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified has been tested by in vitro stimulation of mouse splenocytes.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.5 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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