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          • Primary Antibodies ›
          • IL-1 beta Antibodies

          Invitrogen

          IL-1 beta Monoclonal Antibody (B122), Functional Grade, eBioscience™

          9 Published Figures
          9 References
          View all (138) IL-1 beta antibodies

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          Cite IL-1 beta Monoclonal Antibody (B122), Functional Grade, eBioscience™

          Additional Information:
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          • Published Figures (9)
          IL-1 beta Antibody in Neutralization (Neu)
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          IL-1 beta Antibody in Neutralization (Neu)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          IL-1 beta Antibody (16-7012-38) in Neu

          The Journal of clinical investigation 2023 - Neutralization of circulating IL-1beta attenuates CKD-induced atrial arrhythmogenesis. ( A ) Serum levels of IL-1beta in WT mice 2 and 3 weeks after sham or CKD surgeries. ( B ) Timeline of anti-IL-1beta antibody (5 mg/kg, i.p.) or IgG (as a control) injections into WT-CKD mice. 2/3 Nx, two-thirds nephrectomy. Panel B was created with BioRender.com. ( C and D ) Representative echocardiographic long-axis vie... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          IL-1 beta Antibody in Neutralization (Neu)
          IL-1 beta Antibody in ELISA (ELISA)
          IL-1 beta Antibody in ELISA (ELISA)
          IL-1 beta Antibody in ELISA (ELISA)
          IL-1 beta Antibody in ELISA (ELISA)
          IL-1 beta Antibody in ELISA (ELISA)
          IL-1 beta Antibody in Neutralization (Neu)
          IL-1 beta Antibody in Neutralization (Neu)
          IL-1 beta Antibody in Neutralization (Neu)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          16-7012-38

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          0.125 µg/mL
          View 3 publications 3 publications

          Neutralization (Neu)

          Assay-Dependent
          View 5 publications 5 publications

          Functional Assay (Functional)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Armenian hamster / IgG

          Recommended Isotype Control

          Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          B122

          Conjugate

          Functional Grade Functional Grade Functional Grade
          • Unconjugated
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          5 mg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2866193

          Product Specific Information

          Description: The B122 antibody reacts with mouse and rat interleukin-1beta (IL-1beta), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors.

          Applications Reported:The B122 antibody has been reported for use in cytokine neutralization and ELISA capture.

          Applications Tested: The Functional Grade Purified B122 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-1 beta bioactivity.

          The B122 antibody at 0.125 µg/mL has been found to inhibit by 50% the biological effects of 4.0 ng/mL mouse IL-1 beta in a D10 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

          The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotin anti-mouse Interleukin-1 beta (IL-1 beta) polyclonal (13-7112) antibody for detection and recombinant mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation:Less than 10%, as determined by HPLC.

          Target Information

          Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1b; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta; unnamed protein product

          View more View less

          Gene Aliases: Il-1b; IL-1beta; IL-1F2

          View more View less

          UniProt ID: (Mouse) P10749

          View more View less

          Entrez Gene ID: (Rat) 24494, (Mouse) 16176

          View more View less

          Function(s)
          cytokine activity interleukin-1 receptor binding integrin binding protein domain specific binding receptor agonist activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter fever generation response to hypoxia response to amphetamine chronic inflammatory response to antigenic stimulus inflammatory response immune response positive regulation of cytosolic calcium ion concentration response to nutrient learning or memory memory positive regulation of cell proliferation negative regulation of cell proliferation response to xenobiotic stimulus response to carbohydrate response to ozone response to gamma radiation vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of platelet-derived growth factor receptor signaling pathway positive regulation of epithelial to mesenchymal transition positive regulation of macrophage derived foam cell differentiation negative regulation of glucose transport negative regulation of glutamate secretion cytokine-mediated signaling pathway hyaluronan biosynthetic process positive regulation of cell migration neutrophil chemotaxis positive regulation of prostaglandin biosynthetic process positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to estradiol response to lipopolysaccharide positive regulation of chemokine production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of stress-activated MAPK cascade regulation of organic acid transport positive regulation of immature T cell proliferation in thymus response to ATP response to vitamin D response to L-ascorbic acid positive regulation of heterotypic cell-cell adhesion social behavior ectopic germ cell programmed cell death response to stilbenoid response to isolation stress response to immobilization stress positive regulation of T cell proliferation positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAPK cascade positive regulation of MAPK cascade response to peptide hormone positive regulation of JUN kinase activity positive regulation of neuron apoptotic process positive regulation of nitric oxide biosynthetic process response to ethanol negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of complement activation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway astrocyte activation positive regulation of astrocyte differentiation regulation of defense response to virus by host positive regulation of inflammatory response regulation of neurogenesis negative regulation of neurogenesis regulation of insulin secretion negative regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis response to glucocorticoid positive regulation of protein kinase B signaling positive regulation of glial cell proliferation negative regulation of adiponectin secretion regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade monocyte aggregation interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to antibiotic cellular response to mechanical stimulus cellular response to glucose stimulus cellular response to lipid cellular response to fatty acid cellular response to xenobiotic stimulus response to dexamethasone positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand cellular response to interleukin-17 positive regulation of p38MAPK cascade positive regulation of NIK/NF-kappaB signaling positive regulation of RNA biosynthetic process regulation of establishment of endothelial barrier negative regulation of secretion by cell negative regulation of gap junction assembly response to phorbol 13-acetate 12-myristate response to hydrogen sulfide positive regulation of occluding junction disassembly negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation positive regulation of T-helper 1 cell cytokine production negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.

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