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Invitrogen
IL-2 Monoclonal Antibody (AB12-3G4), Functional Grade, eBioscience™
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Product Details
16-7027-38
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AB12-3G4
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
5 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This AB12-3G4 monoclonal antibody reacts with and inhibits the bioactivity of human IL-2. IL-2 is a 15 kDa cytokine expressed by B cells, T cells, and dendritic cells. IL-2 signals via a heterotrimeric receptor (CD25) and results in the activation of Jak/STAT pathways. IL-2 plays a critical role in the proliferation, activation, and differentiation of T cells. The development of CD4+CD25+ regulatory T cells is especially dependent on the presence of IL-2, making it an important immunoregulatory cytokine.
Applications Reported: This AB12-3G4 monoclonal antibody inhibits the bioactivity of human IL-2.
Applications Tested: The ND50 of AB12-3G4, as determined by the neutralization of Human IL-2-induced proliferation of the CTLL-2 cell line, is less than or equal to 60 ng/mL in the presence of 10 ng/mL Human IL-2 Recombinant Protein. The neutralization dose of this antibody will vary depending on cell type, assay method, and cytokine concentration.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aldesleukin; cytokine; H-IL-2; il 2; ILN; Interleukin; interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; T cell growth factor; T-cell growth factor (TCGF)
Gene Aliases: IL-2; lymphokine; TCGF
UniProt ID: (Human) P60568
Entrez Gene ID: (Human) 3558
leukocyte activation involved in immune response
positive regulation of immunoglobulin production
negative regulation of B cell apoptotic process
immune response
cell adhesion
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
JAK-STAT cascade
cell-cell signaling
positive regulation of cell proliferation
natural killer cell activation
T cell differentiation
positive regulation of cell growth
positive regulation of B cell proliferation
positive regulation of interleukin-17 production
positive regulation of tissue remodeling
interleukin-2-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
negative regulation of apoptotic process
response to ethanol
positive regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
positive regulation of lymphocyte differentiation
positive regulation of inflammatory response
positive regulation of dendritic spine development
positive regulation of leukocyte proliferation
receptor signaling pathway via STAT
positive regulation of plasma cell differentiation
response to tacrolimus
positive regulation of leukocyte differentiation
negative regulation of T-helper 17 cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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