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Invitrogen
IL-10 Monoclonal Antibody (JES5-2A5), Functional Grade, eBioscience™
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FIGURE: 1 / 4
IL-10 Antibody (16-7102-38) in ELISA
Disease models & mechanisms 2016 -
Fig. 6. Elevated IL10 expression and STAT3 activation in ADPKD kidney macrophages. (A) Elevated IL10 expression in human ADPKD kidneys. RNA was isolated from NHKs and non-cystic regions of ADPKD kidneys (three of each). IL10 expression was analyzed by performing qRT-PCR. The line indicates the mean relative value. (B) IL-10 is present in ADPKD cyst fluid. IL-10 in pooled cyst fluid collected from ADPKD kidn... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-7102-38
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
JES5-2A5
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
5 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10).The JES5-2A5 antibody is a neutralizing antibody.Mouse IL-10 is an 18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF).In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10.IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.
Applications Reported:The JES5-2A5 antibody has been reported for use in ELISA, ELISPOT, neutralization of IL-10 bioactivity, and intracellular staining for flow cytometric analysis.
Applications Tested: The Functional Grade Purified JES5-2A5 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-10 bioactivity.
The JES5-2A5 antibody at 0.004 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-10 in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.
The biotinylated JES5-2A5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse interleukin-10 (IL-10) in combination with the purified JES5-16E3 (14-7101) antibody for capture and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine synthesis inhibitory factor; H-IL-10; il 10; ILN; interferon 2a; Interleukin; interleukin 10 precursor; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; unnamed protein product
Gene Aliases: CSIF; If2a; Il-10
UniProt ID: (Mouse) P18893
Entrez Gene ID: (Mouse) 16153
negative regulation of cytokine production
positive regulation of cytokine production
positive regulation of endothelial cell proliferation
response to molecule of bacterial origin
positive regulation of immunoglobulin production
negative regulation of cytokine production involved in immune response
negative regulation of chronic inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
immune response
negative regulation of cell proliferation
response to xenobiotic stimulus
regulation of gene expression
negative regulation of autophagy
response to activity
response to inactivity
negative regulation of myeloid dendritic cell activation
negative regulation of B cell proliferation
response to lipopolysaccharide
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
response to insulin
negative regulation of heterotypic cell-cell adhesion
response to carbon monoxide
cellular response to hepatocyte growth factor stimulus
defense response to bacterium
defense response to protozoan
positive regulation of macrophage activation
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
negative regulation of nitric oxide biosynthetic process
T-helper 1 cell differentiation
negative regulation of MHC class II biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of cell cycle
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
negative regulation of inflammatory response
regulation of synapse organization
regulation of B cell activation
negative regulation of membrane protein ectodomain proteolysis
response to glucocorticoid
negative regulation of cytokine activity
branching involved in labyrinthine layer morphogenesis
cellular response to lipopolysaccharide
cellular response to estradiol stimulus
liver regeneration
interleukin-10-mediated signaling pathway
positive regulation of plasma cell differentiation
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of response to wounding
negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
positive regulation of sprouting angiogenesis
negative regulation of vascular smooth muscle cell proliferation
negative regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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