pCMV-Gaussia-Dura Luc Vector for Luciferase Assays

Catalog number: 16191

Thermo Scientific™  Related applications: Protein Assays and Analysis | Protein Expression

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Description

The Thermo Scientific pCMV-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the strong Cytomegalovirus (CMV) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pCMV-Gaussia-Dura Luc vector:

• Strong (CMV) constitutive promoter for co-transfection and normalization
• Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein that enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The parent pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

Related Products
pMCS-Gaussia-Dura Luc Vector for Luciferase Assays
pTK-Gaussia-Dura Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Reporter Assays
Reporter: Gaussia Dura Luciferase
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Puromycin
Product Size: 10 µg

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

Documents

Manuals & protocols

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