Pierce™ Firefly Luc One-Step Glow Assay Kit, 1000 Reaction Kit - FAQs

All Luciferase Glow Assay Working Solutions should be stored protected from light.

Renilla Luciferase Glow Assay Working Solution must be stored at room temperature (20-25°C) before use and is stable for up to 8 hours at room temperature.

Gaussia Luciferase Glow Assay Working Solution must be stored at room temperature (20-25°C) before use and is stable for up to 4 hours at room temperature. 

Cypridina Luciferase Glow Assay Working Solution must be stored at room temperature (20-25°C) before use and is stable for up to 2 hours at room temperature.

Firefly Luciferase Glow Assay Working Solution must be stored at room temperature (20-25°C) before use and is stable for 4 hours at room temperature. For long term use, store at -20 degrees C for up to 2 months.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

TurboLuc Luciferase One-Step Glow Assay Kit measures activity in mammalian cells with a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications. Several features of the TurboLuc Luciferase One-Step Glow Assay Kit make it suitable for luminometers without injectors and for HTS applications. The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation. Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate containing the transfected, treated cells. The stable signal output (glow enzyme kinetics) of this system provides flexibility with regard to lead time before read. When used with Thermo Scientific TurboLuc 16 Luciferase vectors, the TurboLuc Luciferase One-Step Glow Assay Kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.