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NeoBiotechnologies
DNMT3A/DNA Methyltransferase 3 Alpha Monoclonal Antibody (PCRP-DNMT3A-1E2)
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Product Details
1788-MSM1-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
PCRP-DNMT3A-1E2
Immunogen
Recombinant human full-length DNMT3A protein
Conjugate
Form
Liquid
Concentration
200 µg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
Target Information
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AU015806; Cysteine methyltransferase DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; Dnmt3a; W91664
Gene Aliases: DNMT3A; DNMT3A2; HESJAS; M.HsaIIIA; TBRS
UniProt ID: (Human) Q9Y6K1
Entrez Gene ID: (Human) 1788
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
transcription corepressor activity
DNA (cytosine-5-)-methyltransferase activity
protein binding
methyltransferase activity
zinc ion binding
transferase activity
identical protein binding
unmethylated CpG binding
metal ion binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
lncRNA binding
protein-cysteine methyltransferase activity
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
methylation-dependent chromatin silencing
spermatogenesis
response to xenobiotic stimulus
response to toxic substance
post-embryonic development
response to ionizing radiation
response to lead ion
regulation of gene expression
neuron differentiation
chromatin silencing by small RNA
heterochromatin assembly
response to nutrient levels
methylation
response to estradiol
response to vitamin A
response to cocaine
DNA methylation involved in embryo development
hypermethylation of CpG island
response to ethanol
negative regulation of transcription, DNA-templated
cellular response to amino acid stimulus
cellular response to ethanol
cellular response to hypoxia
genetic imprinting
hepatocyte apoptotic process
autosome genomic imprinting
transposable element silencing by piRNA-mediated DNA methylation
positive regulation of cellular response to hypoxia
cellular response to bisphenol A
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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