Invitrogen™

M-MLV Reverse Transkriptase-Puffer

Wird in 5X Konzentration geliefert

Katalognummer	Menge
18057018	1 ml

Katalognummer 18057018

Preis (EUR)

67,25

Menge:

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

67,25

M-MLV Reverse Transkriptase-Puffer wird als 5X Puffer [250 mM Tris-HCl (pH 8,3), 375 mM KCl, 15 mM MgCl₂] mit 500 µl 0,1 M DTT geliefert. Geliefert mit M-MLV, SuperScript™, SuperScript™ II und SuperScript™ III Reverse Transkriptasen.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Enthält

• M-MLV Reverse Transkriptase-Puffer (1 ml)
• 0,1 M DTT (500 μl)

Lagerung bei –20 °C

Konzentration5X

Zur Verwendung mit (Anwendung)Reverse Transkription

Menge1 ml

Unit SizeEach

Häufig gestellte Fragen (FAQ)

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

What is the highest temperature that MMLV, SuperScript II, SuperScript III, or SuperScript IV RTs can be used?

The optimal temperature for for MMLV is 42 degrees C. The optimal temperature for SuperScript II RT is 42 degrees C, and can be used up to 50 degrees C. The optimal temperature for SuperScript III RT is 50 degrees C, and can be used up to 55 degrees C. For some qRT-PCR reactions where gene-specific primers are used, you can do the RT reaction at 60 degrees C. The optimal temperature for SuperScript IV RT is 50 degrees C, but can be used up to 65 degrees C.

Can I use elevated temperatures for reverse transcription with Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase?

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is not well-suited for elevated temperatures. Typically, M-MLV RT operates optimally at temperatures around 37 degrees C to 42 degrees C.

To perform reverse transcription at elevated temperatures (e.g., 50 degrees C or higher), consider using thermostable reverse transcriptases such as SuperScript IV or Maxima H Minus, which are engineered to perform efficiently at higher temperatures.