Citations & References (2)

Invitrogen™

Taq DNA Polymerase PCR Buffer (10X)

Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 mL of 50 mM MgCl₂.

Catalog Number	Quantity
18067017	2 x 1 mL

Catalog number 18067017

Price (MXN)

2,020.78

Quantity:

2 x 1 mL

Request bulk or custom format

Price (MXN)

2,020.78

Taq DNA Polymerase PCR Buffer is a 10X buffer supplied with 1 mL of 50 mM MgCl₂. It is included with Platinum Taq, Taq, and the SuperScript First-Strand Synthesis System for RT-PCR. This buffer includes 200 mM Tris HCl (pH 8.4) and 500 mM KCl. It is available here to supplement kits where additional buffer may be needed based on protocol variations.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Name or MaterialDNA Polymerase PCR Buffer

Includes• 10X Taq DNA Polymerase PCR Buffer, 2 x 1 mL
• 50 mM MgCl₂, 1 mL

Store at -20°C.
Guaranteed stable for 6 months when properly stored.

Concentration10X

For Use With (Application)Standard PCR

Quantity2 x 1 mL

Unit SizeEach

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all Taq DNA Polymerase PCR Buffer (10X) FAQs

Citations & References (2)

Citations & References

Abstract

Association between CYP2C9 genetic variants and anticoagulation-related outcomes during warfarin therapy.

Authors: Higashi Mitchell K; Veenstra David L; Kondo L Midori; Wittkowsky Ann K; Srinouanprachanh Sengkeo L; Farin Fred M; Rettie Allan E;

Journal:JAMA

PubMed ID:11926893

Edited for space constraints: Warfarin is a commonly used anticoagulant that requires careful clinical management to balance the risks of overanticoagulation and bleeding with those of underanticoagulation and clotting. The principal enzyme involved in warfarin metabolism is CYP2C9, and 2 relatively common variant forms with reduced activity have been identified, ... More

Neutrophil-derived glutamate regulates vascular endothelial barrier function.

Authors: Collard Charles D; Park Kellie A; Montalto Michael C; Alapati Sailaja; Buras Jon A; Stahl Gregory L; Colgan Sean P;

Journal:J Biol Chem

PubMed ID:11847215

Endothelial barrier function is altered by the release of soluble polymorphonuclear leukocyte (PMN)-derived mediators during inflammatory states. However, endogenous pathways to describe such changes are only recently appreciated. Using an in vitro endothelial paracellular permeability model, cell-free supernatants from formylmethionylleucylphenylalanine-stimulated PMNs were observed to significantly alter endothelial permeability. Biophysical and ... More