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Invitrogen™

SuperScript™ III Reverse Transcriptase

Catalog number: 18080044
Invitrogen™

SuperScript™ III Reverse Transcriptase

Catalog number: 18080044
Catalog Number
18080044
also known as 18080-044
Unit Size
10,000 units
Price (USD)
Availability
-
Quantity
Catalog Number
18080093
also known as 18080-093
Unit Size
2,000 units
Price (USD)
Availability
-
Quantity
Catalog Number
18080085
also known as 18080-085
Unit Size
4 x 10,000 units
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
18080044
also known as 18080-044
10,000 units
-
18080093
also known as 18080-093
2,000 units
-
18080085
also known as 18080-085
4 x 10,000 units
-
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Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.

SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E. coli containing the modified pol gene of Moloney Murine Leukemia Virus.

Features of SuperScript III Reverse Transcriptase include:
• First-strand cDNA may be synthesized at temperatures up to 55°C
• cDNA can be generated from 100 bp to >12 kb
• Increased specificity
• High yields of cDNA and full-length products

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Stand-alone Enzyme
GC-Rich PCR Performance
High
Reaction Speed
30 to 50 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
50°C
Quantity
10,000 Units
Reverse Transcriptase
SuperScript III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
Concentration
200 U/μL
Final Product Type
First-Strand cDNA
No. of Reactions
50 Reactions
Reaction Format
Separate components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 12.3 kb
Starting Material
RNA

Contents & Storage

• SuperScript™ III Reverse Transcriptase, 50 μL (10,000 units at 200 U/μL)
• 5X First-strand buffer, 1 mL
• DTT, 500 μL (100 mM)

Store at –20°C.

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