Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Format
Tube
GC-Rich PCR Performance
High
PCR Method
1-step RT-qPCR, 2-step RT-qPCR
Reaction Speed
30 to 50 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
50°C
Quantity
10,000 Units
Reverse Transcriptase
SuperScript III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
Concentration
200 U/μL
Final Product Type
First-Strand cDNA
No. of Reactions
50 Reactions
Reaction Format
Separate components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 12.3 kb
Starting Material
RNA
Volume
50 μL
Contents & Storage
• SuperScript™ III Reverse Transcriptase, 1 x 10,000 units (200 U/μL) • 5X First-strand buffer, 1 mL • DTT, 500 μL (100 mM)