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      SuperScript™ III Reverse Transcriptase

      SuperScript™ III Reverse Transcriptase

      Numéro de catalogue:  18080044

      Invitrogen™  Applications liées: Reverse Transcription

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      Description

      Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.

      SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.

      Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Optimal Reaction Temperature: 50° C
      Final Product Size(s): 12.3 kb or less
      Reverse Transcriptase: SuperScript™ III
      Ribonuclease H Activity: Reduced
      Concentration: 200 U⁄µl
      Enzyme Function: RNA dependent DNA Polymerase
      Form: Frozen
      Format: Tube(s)
      Number of Reactions: 50 Reactions
      Product Line: SuperScript™
      Product Size: 10,000 units
      Quantity: 10,000 units
      Sensitivity: Medium
      Shipping Condition: Dry Ice
      Template: ssRNA

      Contenu et stockage

      SuperScript® III Reverse Transcriptase (10,000 units total, at 200U/µL) is supplied with a vial (1 mL) of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial (500 µL) of 100 mM DTT. Store at -20°C.

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