SuperScript™ III Reverse Transcriptase
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For superior cDNA synthesis upgrade to SuperScript IV Reverse Transcriptase
Invitrogen™
SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutationsRead more
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Catalog NumberNo. of Reactions
1808009310 Reactions
1808004450 Reactions
18080085200 Reactions
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Catalog number 18080093
Price (USD)/ 2,000 units
117.65
Online exclusive
126.00 
Save 8.35 (7%)
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No. of Reactions:
10 Reactions
Request bulk or custom format
Price (USD)/ 2,000 units
117.65
Online exclusive
126.00 
Save 8.35 (7%)
Add to cart
SuperScript™ III Reverse Transcriptase
Catalog number18080093
Price (USD)/ 2,000 units
117.65
Online exclusive
126.00 
Save 8.35 (7%)
-
Add to cart

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.

SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E. coli containing the modified pol gene of Moloney Murine Leukemia Virus.

Features of SuperScript III Reverse Transcriptase include:
• First-strand cDNA may be synthesized at temperatures up to 55°C
• cDNA can be generated from 100 bp to >12 kb
• Increased specificity
• High yields of cDNA and full-length products

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone Enzyme
GC-Rich PCR PerformanceHigh
Reaction Speed30 to 50 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Quantity2,000 Units
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Concentration200 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions10 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 12.3 kb
Starting MaterialRNA
Unit Size2,000 units
Contents & Storage

• SuperScript™ III Reverse Transcriptase, 10 μL (2,000 units at 200 U/μL)
• 5X First-strand buffer, 1 mL
• DTT, 500 μL (100 mM)

Store at –20°C.