BioPrime™ Total Genomic Labeling System

Catalog number:  18097010

 Related applications:

Chromatin Biology

| Genotyping & Genomic Profiling

| Nucleic Acid Labeling & Oligo Synthesis

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The BioPrime® Total Genomic Labeling Systems are complete genomic DNA labeling kits designed for use in array comparative genomic hybridization applications (aCGH) that allow for better call rates with precious samples. The BioPrime® Total Genomic Labeling Systems offer users the following advantages:

- Higher signal to noise with Alexa Fluor® 3 and 5 dyes
- Less channel bias due to novel reaction formulation
- Widest range of input material (50ng - 3µg)
- Simplified workflow with master mix formulation

These complete, all-inclusive kits are optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component limited reaction allow for consistent, robust DNA yields of ~ 8 µg with as little as 50 ng of genomic DNA input. An optimized dye labeled nucleotide mix with new dNTP linkers, novel, application-specific Alexa Fluor® 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor® 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. New master mix formulation streamlines reaction set-ups increasing consistency. Complete kits are batch tested to assure quality in performance from lot to lot.
Contents and Storage:
The simplified kits contains Alexa Fluor® 3 and Alexa Fluor® 5 2x reaction mix, Exo-Klenow fragment (40 U/ µl), 5 mM EDTA, TE Buffer, and control DNA (Salmon Sperm). Store at -80°C for long term storage. The 2X Reaction Mixes may be stored at +4°C for up to 4 weeks and should be protected from light. The BioPrime® Total Genomic Labeling Systems also contain PurelinkTM Genomic DNA purification columns and purification buffers (the BioPrime® Total Genomic Labeling Module does not). Store at room temperature. All components are guaranteed for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Fluorescent
Final Product: Probes (Labeled DNA)
Includes Label or Dye: Yes
Label or Dye: Alexa Fluor® 5, Alexa Fluor™ 3
Labeling Method: Direct Labeling
Labeling Target: Genomic DNA
Product Line: BioPrime®
Product Size: 10 reactions


Manuals & protocols