BioPrime® Total Genomic Labeling System

Catalog number: 18097011

Invitrogen™  Related applications: Chromatin Biology | Genotyping & Genomic Profiling | Nucleic Acid Labeling & Oligo Synthesis

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Description

The BioPrime® Total Genomic Labeling System is a complete genomic DNA labeling system designed for use in array comparative genomic hybridization applications (aCGH) that allows for better call rates with precious samples.

Note: The BioPrime® Total Genomic Labeling System includes PurelinkTM Genomic DNA purification columns and purification buffers. The BioPrime® Total Genomic Labeling Module does not.

The BioPrime® Total Genomic Labeling System offers users the following advantages:

- Higher signal to noise with Alexa Fluor® 3 and 5 dyes
- Less channel bias due to novel reaction formulation
- Widest range of input material (50 ng - 3 µg)
- Simplified workflow with master mix formulation

This complete, all-inclusive kit is optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component-limited reaction allows for consistent, robust DNA yields of ~ 8 µg with as little as 50 ng of genomic DNA input. An optimized dye-labeled nucleotide mix with new dNTP linkers, novel application-specific Alexa Fluor® 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor® 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. New master mix formulation streamlines reaction set-ups, increasing consistency. Complete kits are batch tested to assure quality in performance from lot to lot.

Contents and Storage:
The BioPrime® Total Genomic Labeling System contains Alexa Fluor® 3 and Alexa Fluor® 5 2x reaction mix, Exo-Klenow fragment (40 U/ µl), 5 mM EDTA, TE Buffer, and control DNA (Salmon Sperm). Store at -80°C for long term storage. The 2X Reaction Mixes may be stored at +4°C for up to 4 weeks and should be protected from light. The BioPrime® Total Genomic Labeling System also contains PurelinkTM Genomic DNA purification columns and purification buffers (the BioPrime® Total Genomic Labeling Module does not). Store at room temperature. All components are guaranteed for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
Final Product: Probes (Labeled DNA)
Includes Label or Dye: Yes
Label or Dye: Alexa Fluor® 5, Alexa Fluor™ 3
Labeling Method: Direct Labeling
Labeling Target: Genomic DNA
Product Line: BioPrime®
Product Size: 30 reactions

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