Nick Translation System

The Nick Translation System is ideal for both radioactive and non-radioactive labeling of DNA. The Nick Translation System:• Provides fiveRead more

Catalog Number	Quantity
18160010	50 Reactions

Catalog number 18160010

Price (MXN)

Quantity:

50 Reactions

The Nick Translation System is ideal for both radioactive and non-radioactive labeling of DNA. The Nick Translation System:

• Provides five pre-mixed nucleotide solutions for flexibility in labeled nucleotide incorporation
• Yields >10⁸ cpm/μg control DNA using [ - ³² P]-dCTP
• Labels 1 μg of DNA in one reaction

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Includes Label or DyeNo

Labeling MethodDirect Labeling

Product TypeNick Translation System

Quantity50 Reactions

Shipping ConditionWet Ice

Detection MethodRadioactive, Fluorescence

Final Product TypeProbes (Labeled DNA)

Labeling TargetDNA (General)

Unit SizeEach

Contents & Storage

The Nick Translation System includes an enzyme mix containing DNA Polymerase I and DNase I, dNTPs, control DNA, stop buffer, and distilled water. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

Are labeled nucleotides included in the Nick Translation System?

No, labeled nucleotides are not included.

Citations & References (1)

Citations & References

Abstract

Mastitis increases mammary mRNA abundance of beta-defensin 5, toll-like-receptor 2 (TLR2), and TLR4 but not TLR9 in cattle.

Authors:Goldammer T, Zerbe H, Molenaar A, Schuberth HJ, Brunner RM, Kata SR, Seyfert HM,

Journal:Clin Diagn Lab Immunol

PubMed ID:14715566

Coordination of the primary defense mechanisms against pathogens relies on the appropriate expression of pathogen recognition receptors (PRRs) triggering the early release of effector molecules of the innate immune system. To analyze the impact of this system on the counteraction of infections of the mammary gland (mastitis), we characterized the ... More