DNA Polymerase I/DNase I

Catalog number: 18162016

Invitrogen™  Related applications: Restriction Enzyme Cloning

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Description

DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA.

Application:
Labeling DNA with either radiolabeled or biotinylated nucleotides.

Source:
DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964.

Performance and Quality Testing:
Performance tested in nick translation reaction.

Unit Definition:
One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Unit Reaction Conditions:
50 mM potassium phosphate (pH 7.5), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 µg/ml template•primer, 32 µM dTTP, 69 nM [3H]dTTP, and enzyme in 100 µl for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity: 3' - 5', 5' - 3'
Hot Start: No
Polymerase: DNA Polymerase I
Product Size: 250 units
Shipping Condition: Wet Ice

Contents & storage

DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.

Documents

Manuals & protocols