Thermo Scientific Pierce CDI-Activated Agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilization of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

Pierce CDI-activated Resin is porous, beaded agarose chromatography resin that is activated with carbonyl diimidazole groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilization of many kinds of ligands. Once a molecule is immobilized, the prepared affinity resin can be used multiple times in typical affinity purification procedures.

Features of CDI-activated Agarose Resin:

• Reliable coupling chemistry—immobilization occurs through the reaction of N-nucleophiles with 1,1' -carbonyldiimidazole groups of the resin to form a stable, uncharged N-alkylcarbamate linkages
• Easy-to-use—no secondary reagents needed; just wash equilibrate resin in alkaline coupling buffer and add ligand; reaction proceeds spontaneously
• Stable activation—half-life of hydrolysis is much longer than hydroxysuccinimide ester activations, making immobilization reactions simpler to prepare and control; simplifies filtration and washing before adding a ligand or protein
• Well-defined coupling conditions—reaction is most efficient with primary amines at pH 9 to 11

Properties of CDI-activated Agarose:

• Resin—crosslinked 6% beaded agarose
• Highly activated—at least 50 µMol 1,1'-carbonyldiimidazole (CDI) groups per milliliter of resin
• Convenient form—supplied as stabilized, 50% slurry in acetone

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