Pierce™ CDI-activated Agarose Resin

Catalog number: 20259

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

Thermo Scientific Pierce CDI-Activated Agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilization of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

Pierce CDI-activated Resin is porous, beaded agarose chromatography resin that is activated with carbonyl diimidazole groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilization of many kinds of ligands. Once a molecule is immobilized, the prepared affinity resin can be used multiple times in typical affinity purification procedures.

Features of CDI-activated Agarose Resin:

Reliable coupling chemistry—immobilization occurs through the reaction of N-nucleophiles with 1,1' -carbonyldiimidazole groups of the resin to form a stable, uncharged N-alkylcarbamate linkages
Easy-to-use—no secondary reagents needed; just wash equilibrate resin in alkaline coupling buffer and add ligand; reaction proceeds spontaneously
Stable activation—half-life of hydrolysis is much longer than hydroxysuccinimide ester activations, making immobilization reactions simpler to prepare and control; simplifies filtration and washing before adding a ligand or protein
Well-defined coupling conditions—reaction is most efficient with primary amines at pH 9 to 11

Properties of CDI-activated Agarose:

Resin—crosslinked 6% beaded agarose
Highly activated—at least 50 µMol 1,1'-carbonyldiimidazole (CDI) groups per milliliter of resin
Convenient form—supplied as stabilized, 50% slurry in acetone

Related Products
AminoLink™ Plus Coupling Resin
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Affinity, Agarose Resin
Format: Bottle(s)
Immobilized Phase: Carbonyl Diimidazole
Product Size: 10 mL

Contents & storage

Store at 4°C.

Documents

Manuals & protocols