Thermo Scientific™

Pierce™ High Capacity Streptavidin Agarose

Catalog number: 20359
Thermo Scientific™

Pierce™ High Capacity Streptavidin Agarose

Catalog number: 20359
Catalog Number
20359
Unit Size
5 mL
Price (USD)
Price: 588.00
Online price: 550.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
2 mL
Price (USD)
Price: 372.00
Online price: 347.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
10 mL
Price (USD)
Price: 1,044.00
Online price: 973.65
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Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
203595 mL
Price: 588.00
Online price: 550.65
Your price:
-
203572 mL
Price: 372.00
Online price: 347.65
Your price:
-
2036110 mL
Price: 1,044.00
Online price: 973.65
Your price:
-
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Thermo Scientific Pierce High Capacity Streptavidin Agarose is a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads.

Features of High Capacity Streptavidin Agarose:

Streptavidin—purified recombinant streptavidin protein (53kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
Agarose resin—support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity—this variety of beads has a dense load of immobilized streptavidin, providing a binding capacity greater than 10 mg of biotinylated BSA per mL of resin
Two formats—available in several bottle sizes of resin slurry and as packed 1 mL and 5 mL chromatography cartridges

Applications:
• Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies
• Isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent
• Isolation of cell-surface glycoproteins using biotinylated Concanavalin A
• Recovery of single-stranded biotinylated DNA for dideoxy sequencing
• Streptavidin-coated plates used for direct solid-phase sequencing
In vitro mutagenesis with streptavidin-coated plates
• DNA fingerprinting with streptavidin-coated plates

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar)

Pierce High Capacity Streptavidin Agarose consists of purified recombinant streptavidin that has been covalently immobilized at a high density onto high-quality crosslinked 6% beaded agarose. Streptavidin is a tetrameric biotin-binding protein. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin.

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

High-Capacity Streptavidin Agarose beads have a dense load of immobilized streptavidin, providing a binding capacity greater than 10 mg of biotinylated BSA per mL of resin. This corresponds to 2 to 3 times higher biotin-binding capacity than products from other suppliers, allowing use of small amounts of resin for experiments.

Related Products
Pierce™ High Capacity Streptavidin Chromatography Cartridges, 1 mL
Pierce™ High Capacity Streptavidin Chromatography Cartridge, 5 mL
Pierce™ Streptavidin Agarose
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity (Metric)
10 mg Biotinylated BSA Binding per mL Resin
Particle Size
45 to 165 μm
Product Line
Pierce™
Quantity
5 mL
Protein Form
Recombinant
Target
Biotin-Tagged Molecule
Type
Agarose
Stationary Phase
Streptavidin
Sufficient For
Binding ≥/= 100 μg Biotin/mL Resin (≥/= 10 mg Biotinylated BSA/mL Resin)
Format
Bottle
Column Type
Affinity, Agarose Resin

Contents & Storage

Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature.

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