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Clean-Blot™ IP Detection Kit (HRP) - FAQs

Clean-Blot™ IP Detection Kit (HRP) - FAQs

View additional product information for Clean-Blot™ IP Detection Kit (HRP) - FAQs (21232)

5 product FAQs found

Can I strip and reprobe my blot after using the Clean-Blot IP Detection reagent?

Yes. When using a chemiluminescent substrate, membranes can be stripped and reprobed similar to probing with secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What substrates are compatible with the Clean-Blot IP Detection reagent?

For best results, use the the Clean-Blot IP Detection with SuperSignal Substrates or Thermo Scientific ECL Western Blotting Substrate. When using other HRP or AP substrates, empirically determine the optimal concentration of the Clean-Blot IP Detection Reagent.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What blocking buffers are compatible with the Clean-Blot IP Detection reagent?

The Clean-Blot IP Detection is compatible with bovine serum albumin, SuperBlock and StartingBlock Blocking Buffers and 5% nonfat milk. Verify compatibility with other blocking buffers by dot-blot analysis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Does the Clean-Blot IP Detection Reagent bind to all antibody classes and subclasses?

The reagent binds to IgG from a wide range of species: bovine IgG2, goat IgG2, human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b, IgG3, rat IgG2c, sheep IgG2, pig, dog and cat. It will not bind to rat IgG2a and IgG2b or mouse IgG1. If in doubt whether this detection reagent will bind to a specific antibody, perform a dot-blot analysis before the experiment.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How does the Clean-Blot IP Detection Kit work?

This assay uses a HRP conjugate or AP conjugate that binds to native IgG from various host species, allowing clear, specific Western blot detection from IP experiments without interference from denatured IgG. It is used simply as a substitute for secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.