Thermo Scientific™

Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)

Catalog number: 21650
Thermo Scientific™

Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)

Catalog number: 21650
Catalog Number
21650
Unit Size
50 mg
Price (USD)
Price: 530.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2165050 mg
Price: 530.00
Your Price:
-
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Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

Features of Sulfo-LC-SPDP:

Reactive groups: NHS ester and pyridyldithiol
Reactive towards: amino and sulfhydryl groups
• Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
• Water soluble, sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
• Cleavable crosslinker allows separation of crosslinked products
• Sulfo-LC-SPDP crosslinker is not membrane permeable, allowing cell surface labeling
• Compare to other varieties of SPDP-type reagents, including pegylated forms

Sulfo-LC-SPDP is Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

Applications:
• Reversible crosslinking of proteins
• Adding sulfhydryl groups to proteins by attachment to lysine residues
• Protein crosslinking to create antibody-enzyme and other bioconjugates via a two-step reaction
• Attaching haptens to carrier proteins for antibody production
• Preparing immunotoxin conjugates

Specifications:
We manufacture Sulfo-LC-SPDP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-LC-SPDP is tested to meet the following minimum specifications.

• Identity: IR scan shows only peaks characteristic of the structure and functional groups of Sulfo-LC-SPDP crosslinker
• Solubility: ≥ 95 mg/mL in DI water
• Purity: ≥ 90% by quantitative NMR (the highest standard for crosslinker purity)

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
SPDP (succinimidyl 3-(2-pyridyldithio)propionate)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line
Pierce™
Labeling Method
Chemical Labeling
Crosslinker Type
Heterobifunctional
Reactive Moiety
Pyridyldisulfide, Sulfo-NHS Ester
Spacer
Cleavable, Long
Form
Powder
Quantity
50 mg
Solubility
Water
Product Type
Crosslinker

Contents & Storage

Upon receipt store desiccated at -20° C. Product is shipped at ambient temperature.