Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis.
AuthorsKida Y, Morimoto F, Sakaguchi M
JournalJ Cell Biol
PubMed ID18166653
During protein integration into the endoplasmic reticulum, the N-terminal domain preceding the type I signal-anchor sequence is translocated through a translocon. By fusing a streptavidin-binding peptide tag to the N terminus, we created integration intermediates of multispanning membrane proteins. In a cell-free system, N-terminal domain (N-domain) translocation was arrested by ... More
Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis.
AuthorsAuclair JR, Boggio KJ, Petsko GA, Ringe D, Agar JN
JournalProc Natl Acad Sci U S A
PubMed ID21098299
Amyotrophic lateral sclerosis (ALS) is a disorder characterized by the death of both upper and lower motor neurons and by 3- to 5-yr median survival postdiagnosis. The only US Food and Drug Administration-approved drug for the treatment of ALS, Riluzole, has at best, moderate effect on patient survival and quality ... More