Thermo Scientific™

L-Photo-Leucine

Catalog number: 22610
Thermo Scientific™

L-Photo-Leucine

Catalog number: 22610
Catalog Number
22610
Unit Size
100 mg
Price (USD)
Price: 512.00
Online price: 482.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
22610100 mg
Price: 512.00
Online price: 482.65
Your price:
-
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Thermo Scientific Pierce L-Photo-Leucine is an analog of L-leucine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.

Features of L-Photo-Leucine:

In vivo labeling—incorporate photo-reactive group into proteins using normal cellular machinery of live cells
In vivo crosslinking—find interacting proteins in the native cellular environment
Increased specificity—crosslink interacting proteins correctly positioned at their interfaces within protein interaction domains
Efficient recovery—90% protein recovery in cell lysates after crosslinking
Compatible—crosslink proteins expressed in a wide variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3 and C6
Easy to use—reagents are photo-stable under normal laboratory lighting, eliminating the need to work in the dark

Thermo Scientific L-Photo-Leucine is an analog of L-leucine that can be endogenously incorporated into the primary sequence of proteins during synthesis and then ultraviolet light (UV)-activated to covalently crosslink proteins within protein-protein interaction domains in their native environment within live cells. The powerful method enables both stable and transient protein interactions in cells to be studied and characterized without the use of completely foreign chemical crosslinkers and associated reagent solvents during the interaction experiment that can adversely aspects of the cell biology being studied.

When used in combination with specially formulated limiting media that is devoid of leucine, the photo-activatable derivative is treated like a naturally occurring amino acid by the protein synthesis machinery within the cell. As a result, it can be substituted for leucine in the primary sequence of proteins during catabolism and growth. Photo-leucine derivatives contain diazirine rings that activate when exposed to UV light to become reactive intermediates that form covalent bonds with nearby protein side chains and backbones. Naturally associating binding partners within the cell can be instantly trapped by photoactivation of the diazirine-containing proteins in the cultured cells. Crosslinked protein complexes can be detected by decreased mobility on SDS-PAGE followed by western blot detection, size exclusion chromatography, sucrose density gradient sedimentation or mass spectrometry.

Resources:

Photoreactive Amino Acid FAQ

Related Products
L-Photo-Methionine
Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity
Nonselective-NA (Metabolic labeling)
Cleavable
No
Description
L-Photo-Leucine
Molecular Weight (g/mol)
143.15
PEGylated
No
Spacer Arm Length
0.0 Å
Cell Permeability
Yes
Shipping Condition
Ambient
Product Line
Pierce™
Labeling Method
Chemical Labeling, Metabolic Labeling
Crosslinker Type
Heterobifunctional
Reactive Moiety
Diazirine
Spacer
Short (<10 Å)
Form
Solid
Quantity
100 mg
Solubility
Water
Format
Standard
Water Soluble
Yes
Product Type
Crosslinker

Contents & Storage

Upon receipt store at 4°C protected from light.

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