Invitrogen™

Proteinase K Solution (20 mg/mL), RNA grade

Catalog number: 25530049
Invitrogen™

Proteinase K Solution (20 mg/mL), RNA grade

Catalog number: 25530049
Catalog Number
25530049
Unit Size
5 mL
Price (USD)
Price: 195.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
255300495 mL
Price: 195.00
Your Price:
-
Product Overview
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Additional Information
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Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Liquid
Concentration
20 mg/mL
Purity
DNase-Free, RNase-Free
For Use With (Application)
Chromatin Biology/RNA Extraction/DNA Extraction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Quantity
5 mL
Product Type
Proteinase K

Contents & Storage

Store in freezer (-5 to -30°C).