Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:
• Over a wide pH range—optimal activity between 6.5 and 9.5 • Under denaturing conditions—e.g., in the presence of SDS or urea • In the presence of metal chelating agents—e.g., EDTA • At comparatively high temperatures—optimum digestion temperature is 65°C
Applications Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.
For Research Use Only. Not for use in diagnostic procedures.