CaptureSelect™ IgG-Fc (Multispecies) Magnetic Agarose Beads

CaptureSelect IgG-Fc (Multispecies) Magnetic Agarose Beads are high-capacity, high-throughput magnetic affinity particles for antibody purification using manual or robotic magnetic separators. The CaptureSelect IgG-Fc (multispecies) ligand has been specifically designed to purify IgG through high-affinity binding of the IgG Fc region. These magnetic agarose beads can be used for the purification of IgG from a broad range of species. The tested species and their specificity are shown in the figure below.

Features of CaptureSelect IgG-Fc (multispecies) Magnetic Agarose Beads include:

• One-step IgG purification with high purity, from crude material
• Compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher instruments)
• Short operation time
• No sample dilution required during isolation, repetitive sample loading feasible
• Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: 6% cross-linked magnetized agarose
• Average particle size: 25–30 μm
• Suspension: 25% slurry
• Ligand: CaptureSelect IgG-Fc (multispecies) affinity ligand
• Binding capacity: >4 mg IgG/mL slurry
• Elution conditions: 0.1 M glycine, pH 3.0