iScience 2023 - Generation and molecular analysis of global double GluA1 and GluA3 knockout mice ( Gria1/3 -/- ) and constitutive mutants ( Gria1/3 DeltaFb ) with specific loss of the GluA1 and GluA3 subunits in excitatory forebrain neurons (A) Gria1/3 -/- were generated by matings of GluA1 and GluA3 knockout mice. A three-week-old Gria1/3 -/- mouse raised by a foster mother can be recognized by its smaller body size and i... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

GluR3 Antibody — Figure 4. A , B , Changes in AMPA receptor subunit expression of the AMPA receptor subunits GluR-A, GluR-B, GluR-C, and GluR-D in hippocampus ( A ) and cerebellum ( B ) of wild-type (WT) and GluR-C knock-out (GluR-C -/-) mice from P2 to P90. Total proteins of the hippocampus and the cerebellum were isolated, and for each sample, 10 mug of protein was separated on a 7.5% SDS-PAGE and transferred on nitrocellulose. The glutamate receptor subunits were visualized by selective antibodies and monitored by autoradiography. The alpha-subunit of the Ca 2+ /calmodulin-dependent protein kinase II (alpha-CaMKII) and betaIII-tubulin (betaIII-Tub.) was used as a positive control for developmental controlled gene expression in hippocampus and cerebellum, respectively. Loading was controlled by beta-actin. The experiment was repeated with at least three sets of mice. One representative example for each subunit is given. {KO}

Immunogen image

Immunogen Image GluR3 Monoclonal Antibody (3B3)

Product Details

32-0400

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

1-3 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Non-human primate, Rat

Published species

Mouse, Rat, Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3B3

Immunogen

Fusion protein encompassing the N-terminal region of rat GluR3.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Ambient (domestic); Wet ice (international)

AB_2533059

Target Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AMPA receptor subunit GluA3; AMPA-selective glutamate receptor 3; dJ1171F9.1; gluR-3; glutamate receptor; glutamate receptor (GluR-C); glutamate receptor C; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor, ionotropic, AMPA 3; glutamate receptor, ionotropic, AMPA3 (alpha 3); OTTHUMP00000024262; RP11-349N19.3; unnamed protein product

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Gene Aliases: GluA3; GluR-3; GLUR-C; GLUR-K3; GLUR3; GLURC; iGluR3; MRX94; MRXSW

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UniProt ID: (Human) P42263, (Rat) P19492

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Entrez Gene ID: (Human) 2892, (Rat) 29628

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Function(s)

beta-amyloid binding ionotropic glutamate receptor activity AMPA glutamate receptor activity ion channel activity protein binding ligand-gated ion channel activity glutamate-gated calcium ion channel activity signaling receptor activity ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential ligand-gated calcium channel activity

Process(es)

ion transport glutamate receptor signaling pathway calcium-mediated signaling ion transmembrane transport ionotropic glutamate receptor signaling pathway synaptic transmission, glutamatergic modulation of synaptic transmission protein homotetramerization protein heterotetramerization regulation of postsynaptic membrane potential long-term synaptic potentiation regulation of presynaptic membrane potential regulation of receptor recycling response to lithium ion response to fungicide cellular response to glycine

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GluR3 Monoclonal Antibody (3B3)

GluR3 Antibody (32-0400) in WB

Product Details

32-0400

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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