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Invitrogen
NMDAR1 Monoclonal Antibody (54.1)
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FIGURE: 1 / 46
NMDAR1 Antibody (32-0500) in ICC/IF
Immunofluorescent analysis of MAP2 (green) and NMDAR1 (red) on rat primary Hippocampal neurons (E18) (Product # A15587) cultured for 28 days in the B-27 Plus Neuronal Culture System (Product # A3653401). At day 28 the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% triton x-100 for 30min, and blocked with 1% BSA for 30 min at room temperature. Cells were stained with anti-NMDAR1 antibody (Product # 32-0500) at a dilution of 1:100, and anti-MAP2 (Product # PA5-17646) at a dilution of 1:250, in 1% BSA staining b... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
32-0500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Fish,
Hamster,
Human,
Mouse,
Rat,
Tag
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
54.1
Immunogen
Fusion protein containing sequence from the intracellular loop between transmembrane regions III and IV of NMDAR1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody specifically recognizes the ~103 kDa NMDAR1 protein. Cross-reactivity with other NMDA receptor proteins has not been observed.
Target Information
NMDAR1 encodes a protein that is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Glun1r; glutamate [NMDA] receptor subunit zeta-1; glutamate receptor; glutamate receptor, ionotropic, N-methyl D-aspartate 1; N-methyl-D-aspartate glutamate receptor; N-methyl-D-aspartate receptor channel, subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; neurotransmitter receptor; NMD-R1; NMDA R1 receptor C1 cassette; NMDA receptor 1; NMDZ1; NMZ1; NR1C1; NR1C2; NR1C2'; NR1N1; pGluN1; putative NMDtranscript(altAcc_e2); RP11-350O14.1; unnamed protein product
Gene Aliases: DEE101; GluN1; GluRdelta1; GluRzeta1; hNR1; M100174; MRD8; NDHMSD; NDHMSR; NMD-R1; NMDA1; Nmdar; NMDAR1; NR1; Rgsc174
UniProt ID: (Human) Q05586, (Rat) P35439, (Mouse) P35438
Entrez Gene ID: (Human) 2902, (Rat) 24408, (Mouse) 14810
beta-amyloid binding
NMDA glutamate receptor activity
ion channel activity
calcium channel activity
calcium ion binding
protein binding
calmodulin binding
ligand-gated ion channel activity
ligand-gated sodium channel activity
glycine binding
glutamate binding
transmitter-gated ion channel activity
glutamate-gated calcium ion channel activity
signaling receptor activity
macromolecular complex binding
glycine-gated cation channel activity
ionotropic glutamate receptor activity
receptor binding
cation channel activity
enzyme binding
phosphatase binding
voltage-gated cation channel activity
glutamate receptor binding
ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
ionotropic glutamate receptor binding
ion transport
cation transport
chemical synaptic transmission
brain development
visual learning
positive regulation of calcium ion transport into cytosol
propylene metabolic process
calcium-mediated signaling
ionotropic glutamate receptor signaling pathway
sodium ion transmembrane transport
regulation of membrane potential
response to ethanol
positive regulation of transcription from RNA polymerase II promoter
regulation of synaptic plasticity
regulation of neuronal synaptic plasticity
protein heterotetramerization
positive regulation of synaptic transmission, glutamatergic
calcium ion homeostasis
excitatory postsynaptic potential
calcium ion transmembrane transport
calcium ion transmembrane import into cytosol
cation transmembrane transport
excitatory chemical synaptic transmission
positive regulation of reactive oxygen species biosynthetic process
regulation of cation transmembrane transport
cellular response to beta-amyloid
response to glycine
positive regulation of excitatory postsynaptic potential
conditioned taste aversion
response to hypoxia
startle response
suckling behavior
response to amphetamine
calcium ion transport
cellular calcium ion homeostasis
positive regulation of cytosolic calcium ion concentration
learning or memory
learning
memory
long-term memory
associative learning
adult locomotory behavior
olfactory learning
regulation of cell communication
response to amine
sensory perception of pain
pons maturation
hypothalamus development
cerebral cortex development
social behavior
synaptic transmission, glutamatergic
positive regulation of apoptotic process
response to morphine
regulation of neuron apoptotic process
negative regulation of neuron apoptotic process
regulation of respiratory gaseous exchange
regulation of long-term neuronal synaptic plasticity
regulation of dendrite morphogenesis
regulation of axonogenesis
regulation of synapse organization
neuromuscular process
positive regulation of mitochondrial calcium ion concentration
response to calcium ion
regulation of synapse assembly
regulation of postsynaptic membrane potential
prepulse inhibition
male mating behavior
response to fungicide
protein-containing complex assembly
cellular response to manganese ion
regulation of presynaptic membrane potential
chemical synaptic transmission, postsynaptic
regulation of plasma membrane bounded cell projection organization
positive regulation of Schwann cell migration
positive regulation of dendritic spine maintenance
response to glycoside
protein localization to postsynaptic membrane
cellular response to glycine
rhythmic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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