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Invitrogen
N-cadherin Monoclonal Antibody (3B9)
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FIGURE: 1 / 8
N-cadherin Antibody (33-3900) in ICC/IF
Immunofluorescent analysis of N-Cadherin was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with N-Cadherin Mouse Monoclonal Antibody (Product # 33-3900) at 1 µg - 2 µg in 1% BSA and incubated for 3 hours ... View More
Product Details
33-3900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Pig,
Rat
Published species
Avian,
Dog,
Hamster,
Horse,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Tag,
Zebrafish
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3B9
Immunogen
Recombinant domain corresponding to the intracellular domain of chicken N-Cadherin
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for N-cadherin and does not cross-react with other cadherin family members including P- and E-cadherins.
For IHC with this antibody use formalin-fixed, paraffin-embedded tissue and heat-induced epitope retrieval.
Target Information
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin; neural calcium-dependent cell adhesion molecule; unnamed protein product
Gene Aliases: ACOGS; ADHD8; ARVD14; cadherin-2; CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD
UniProt ID: (Human) P19022, (Chicken) P10288, (Mouse) P15116, (Rat) Q9Z1Y3
Entrez Gene ID: (Pig) 100515322, (Human) 1000, (Chicken) 414745, (Mouse) 12558, (Rat) 83501
RNA binding
calcium ion binding
protein binding
beta-catenin binding
enzyme binding
protein kinase binding
protein phosphatase binding
identical protein binding
alpha-catenin binding
gamma-catenin binding
cadherin binding
metal ion binding
extracellular matrix binding
protein complex binding
nitric-oxide synthase binding
cadherin
cell morphogenesis
type B pancreatic cell development
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
synapse assembly
glial cell differentiation
neural crest cell development
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
telencephalon development
cerebral cortex development
adherens junction organization
detection of muscle stretch
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
striated muscle cell differentiation
radial glial cell differentiation
neuroepithelial cell differentiation
regulation of oligodendrocyte progenitor proliferation
protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
mesenchymal cell migration
synaptic vesicle clustering
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
cell-cell adhesion
regulation of postsynaptic density protein 95 clustering
positive regulation of synaptic vesicle clustering
cartilage condensation
neural tube formation
brain development
negative regulation of cell migration
neural crest cell delamination
regulation of axonogenesis
regulation of synaptic transmission, glutamatergic
limb development
trigeminal ganglion formation
trigeminal ganglion structural organization
cell-cell adhesion via plasma-membrane adhesion molecules
cell aggregation
regulation of signal transduction
single organismal cell-cell adhesion
regulation of myelination
regulation of protein localization
regulation of Rho protein signal transduction
protein heterooligomerization
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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