ICP27 interacts with SRPK1 to mediate HSV splicing inhibition by altering SR protein phosphorylation.
AuthorsSciabica KS, Dai QJ, Sandri-Goldin RM,
JournalEMBO J
PubMed ID12660167
'Infection with some viruses can alter cellular mRNA processing to favor viral gene expression. We present evidence that herpes simplex virus 1 (HSV-1) protein ICP27, which contributes to host shut-off by inhibiting pre-mRNA splicing, interacts with essential splicing factors termed SR proteins and affects their phosphorylation. During HSV-1 infection, phosphorylation ... More
SR splicing factors serve as adapter proteins for TAP-dependent mRNA export.
AuthorsHuang Y, Gattoni R, Stévenin J, Steitz JA,
JournalMol Cell
PubMed ID12667464
'The only mammalian RNA binding adapter proteins known to partner with TAP/NXF1, the primary receptor for general mRNA export, are members of the REF family. We demonstrate that at least three shuttling SR (serine/arginine-rich) proteins interact with the same domain of TAP/NXF1 that binds REFs. Included are 9G8 and SRp20, ... More
Regulation of alternative splicing by SRrp86 and its interacting proteins.
SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and ... More
The cardiotonic steroid digitoxin regulates alternative splicing through depletion of the splicing factors SRSF3 and TRA2B.
AuthorsAnderson ES, Lin CH, Xiao X, Stoilov P, Burge CB, Black DL
JournalRNA
PubMed ID22456266
Modulation of alternative pre-mRNA splicing is a potential approach to therapeutic targeting for a variety of human diseases. We investigated the mechanism by which digitoxin, a member of the cardiotonic steroid class of drugs, regulates alternative splicing. Transcriptome-wide analysis identified a large set of alternative splicing events that change after ... More