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          • Primary Antibodies ›
          • BAX Antibodies

          Invitrogen

          Bax Monoclonal Antibody (6A7)

          6 Published Figures
          15 References
          View all (86) BAX antibodies

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          Cite Bax Monoclonal Antibody (6A7)

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (6)
          Bax Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Bax Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Bax Antibody (33-6600) in IHC (P)

          Immunohistochemistry analysis of Bax showing staining in the cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Bax Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bax Antibody in Western Blot (WB)
          Bax Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bax Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bax Antibody in Immunohistochemistry (IHC)
          Bax Antibody in Immunohistochemistry (IHC)
          Bax Antibody in Immunohistochemistry (IHC)
          Bax Antibody in Immunohistochemistry (IHC)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Bax Monoclonal Antibody (6A7)

          Product Details

          33-6600

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 8 publications 8 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:10-1:100
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          Assay-dependent
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Published species

          Human, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          6A7

          Immunogen

          Synthetic peptide corresponding to a sequence at the N-terminal of the protein that is common to mouse and rat.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533133

          Target Information

          BAX is a members of the Bcl-2 Family and plays an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of BAX and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa). BAX is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX can form homodimers and is also able to heterodimerize with other BCL-2 related proteins.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: apoptosis inducer; Bax zeta; Bcl2-associated X protein; unnamed protein product

          View more View less

          UniProt ID: (Mouse) Q07813

          View more View less

          Entrez Gene ID: (Rat) 24887, (Mouse) 12028

          View more View less

          Function(s)
          protein binding lipid binding channel activity Hsp70 protein binding heat shock protein binding identical protein binding protein homodimerization activity macromolecular complex binding protein heterodimerization activity chaperone binding BH domain binding BH3 domain binding
          Process(es)
          ovarian follicle development response to hypoxia neuron migration leukocyte homeostasis T cell homeostatic proliferation B cell homeostasis B cell apoptotic process kidney development release of cytochrome c from mitochondria blood vessel remodeling myeloid cell homeostasis B cell negative selection B cell homeostatic proliferation positive regulation of B cell apoptotic process response to ischemia glycosphingolipid metabolic process regulation of nitrogen utilization apoptotic process cellular response to DNA damage stimulus mitochondrion organization mitochondrial membrane organization inner mitochondrial membrane organization outer mitochondrial membrane organization germ cell development spermatogenesis nervous system development sex differentiation mitochondrial fusion cell proliferation negative regulation of cell proliferation male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage apoptotic mitochondrial changes response to xenobiotic stimulus fertilization response to wounding response to toxic substance response to salt stress post-embryonic development response to ionizing radiation establishment or maintenance of transmembrane electrochemical gradient response to gamma radiation positive regulation of calcium ion transport into cytosol negative regulation of mitochondrial membrane potential hypothalamus development cerebral cortex development positive regulation of protein complex assembly negative regulation of protein binding negative regulation of endoplasmic reticulum calcium ion concentration release of matrix enzymes from mitochondria negative regulation of peptidyl-serine phosphorylation cellular response to stress regulation of mammary gland epithelial cell proliferation glial cell apoptotic process cellular response to UV limb morphogenesis ectopic germ cell programmed cell death swimming behavior response to cocaine odontogenesis of dentin-containing tooth regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process regulation of neuron apoptotic process negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process mitochondrial fragmentation involved in apoptotic process development of secondary sexual characteristics cellular respiration retinal cell programmed cell death response to copper ion positive regulation of developmental pigmentation negative regulation of fibroblast proliferation spermatid differentiation post-embryonic camera-type eye morphogenesis response to axon injury homeostasis of number of cells homeostasis of number of cells within a tissue protein insertion into mitochondrial membrane positive regulation of release of sequestered calcium ion into cytosol neuron apoptotic process response to corticosterone regulation of cell cycle regulation of mitochondrial membrane potential Sertoli cell proliferation retina development in camera-type eye positive regulation of apoptotic process involved in mammary gland involution vagina development intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress thymocyte apoptotic process cellular response to hydrogen peroxide intrinsic apoptotic signaling pathway by p53 class mediator response to anesthetic positive regulation of release of cytochrome c from mitochondria apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand intrinsic apoptotic signaling pathway execution phase of apoptosis fibril organization cellular response to virus regulation of mitochondrial membrane permeability involved in apoptotic process positive regulation of mitochondrial membrane permeability involved in apoptotic process apoptotic process involved in patterning of blood vessels apoptotic process involved in embryonic digit morphogenesis regulation of mitochondrial membrane permeability involved in programmed necrotic cell death positive regulation of apoptotic DNA fragmentation B cell receptor apoptotic signaling pathway response to insulin-like growth factor stimulus negative regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand positive regulation of intrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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