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Invitrogen
SR Monoclonal Antibody (16H3 (16H3E8))
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
33-9300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Fruit fly,
Human,
Mouse,
Rabbit,
Rat,
Xenopus
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
16H3 (16H3E8)
Immunogen
Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST. The 16H3 epitope has been mapped to a 40-amino acid polypeptide composed almost exclusively of arginine alternating with glutamate and aspartate
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This monoclonal antibody can be used to detect a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40, and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. 16H3 also recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF. The epitope detected by the 16H3 monoclonal antibody has been termed the 'alternating arginine domain' and is composed almost exclusively of argnine alternating with glutamate and aspartate. 16H3 targets are predominantly nuclear, nonnucleolar, and are localized to active sites of polymerase II transcription.
Target Information
SR proteins are a highly conserved family of arginine/serine-rich, spliceosome-associated phosphoproteins essential for metazoan pre-mRNA splicing. SR proteins act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role because they can determine alternative splice site usage in vivo and in vitro. SR proteins appear to be recruited from nuclear 'speckles', in which they are concentrated to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: delayed-early protein HRS; epididymis secretory protein Li 91; epididymis secretory sperm binding protein; insulin-induced growth response protein CL-4; pre-mRNA splicing factor SRp20; pre-mRNA-splicing factor SRP20; pre-mRNA-splicing factor SRP40; pre-mRNA-splicing factor SRP55; pre-mRNA-splicing factor SRP75; putative; RNA binding protein; serine/arginine-rich splicing factor 4; SFRS5; splicing factor SRp55; splicing factor SRp55-1; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich 3 (SRp20); splicing factor, arginine/serine-rich 4; splicing factor, arginine/serine-rich 4 (SRp75); splicing factor, arginine/serine-rich 5; splicing factor, arginine/serine-rich 5 (SRp40, HRS); splicing factor, arginine/serine-rich 6; splicing factor, arginine/serine-rich, 20-kD; splicing factor, arginine/serine-rich, 55 kDa; SR splicing factor 4; SR splicing factor 5; SR splicing factor 6; SRP001LB; unnamed protein product; X16
Gene Aliases: 1210001E11Rik; 5730499P16Rik; B52; HEL-S-91; HRS; MNCb-2616; RGD1561347; SFRS3; SFRS4; SFRS5; SFRS6; SRP20; SRP40; SRP55; SRP55-1; SRP75; X16
UniProt ID: (Bovine) Q3SZR8, (Human) P84103, (Human) Q08170, (Human) Q13243, (Human) Q13247, (Bovine) Q3B7L6, (Mouse) P84104, (Rat) Q09167, (Mouse) O35326, (Mouse) Q3TWW8, (Rat) G3V6S8
Entrez Gene ID: (Dog) 403687, (Chicken) 419815, (Bovine) 540276, (Human) 6428, (Rabbit) 100347748, (Rabbit) 100350179, (Bovine) 614310, (Chicken) 419600, (Dog) 478160, (Human) 6429, (Rabbit) 100340192, (Dog) 490754, (Chicken) 423258, (Human) 6430, (Bovine) 510474, (Human) 6431, (Chicken) 419116, (Bovine) 507828, (Dog) 403667, (Rabbit) 100008654, (Mouse) 20383, (Rat) 361814, (Rat) 362612, (Mouse) 57317, (Rat) 29667, (Mouse) 20384, (Mouse) 67996, (Rat) 362264
mRNA export from nucleus
regulation of mRNA splicing, via spliceosome
cellular response to leukemia inhibitory factor
regulation of alternative mRNA splicing, via spliceosome
RNA processing
primary miRNA processing
RNA splicing, via transesterification reactions
mRNA processing
RNA splicing
response to insulin
negative regulation of mRNA splicing, via spliceosome
liver development
mRNA splice site selection
cellular response to insulin stimulus
positive regulation of RNA splicing
liver regeneration
alternative mRNA splicing, via spliceosome
negative regulation of gene expression
regulation of keratinocyte proliferation
negative regulation of keratinocyte differentiation
positive regulation of epithelial cell proliferation involved in lung morphogenesis
regulation of wound healing
negative regulation of type B pancreatic cell apoptotic process
insulin receptor signaling pathway
biological_process
hematopoietic progenitor cell differentiation
response to wounding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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