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SRSF3 Monoclonal Antibody (1H4G7)

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9 Published Figures

14 References

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Datasheet

Certificates

Protocols

Questions & Answers

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SRSF3 Antibody (33-9400) in WB

Western blot of HeLa cell lysates using Mouse anti-SR Proteins monoclonal Antibody (Product # 33-9400).

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SRSF3 Antibody — Figure 6 Digoxin alters the activity of SR protein kinases and induces modification of a subset of SR proteins. (A) To assess the effect of digoxin on CLK kinase function, HeLa rtTA-HIV-Delta Mls cells were transfected with vectors expressing GFP-tagged CLK1. Twenty-four hours post-transfection, cells were treated with DMSO or 100 nM digoxin. After 24 h of treatment, cells were fixed, stained for SC35/SRSF2 nuclear speckles (Texas Red), and nuclei stained with DAPI. Images are representative of the localization patterns observed from >=5 independent experiments. Magnification 630x. (B, C, D) Western blot analysis of the effect of digoxin treatment on SR proteins. Lysates were harvested from HeLa rtTA-HIV-Delta Mls cells treated with 100 nM digoxin or DMSO and were induced with Dox (+) or left uninduced (-) as described in Fig. 1 . Immunoblots were probed by specific antibody for (B) phospho-SR proteins (1H4) or (C) SRp20, Tra2beta1, 9G8, or SF2/ASF (SF2). (D) Cell lysates from above were dephosphorylated by treatment with (+) and without (-) calf intestinal alkaline phosphatase (CIP), then immunoblotted with a SRp20-specific antibody. Arrows on the right indicate the protein specie(s) detected and their modified/unmodified isoform(s). Anti-tubulin (Anti-Tub.) blots for each experiment served as an internal loading control for the relative amount of protein lysate per lane. Immunoblots are representative of >=4 independent trials. {TM}

Immunogen image

Immunogen Image SRSF3 Monoclonal Antibody (1H4G7)

Product Details

33-9400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Xenopus

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1H4G7

Immunogen

Mass-isolated nuclei from Xenopus Laevis oocytes.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533150

Target Information

SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: epididymis secretory sperm binding protein; pre-mRNA splicing factor SRp20; pre-mRNA-splicing factor SRP20; RNA binding protein; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD; unnamed protein product

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Gene Aliases: SFRS3; SRp20

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UniProt ID: (Human) P84103

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Entrez Gene ID: (Human) 6428

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Function(s)

nucleic acid binding RNA binding mRNA binding protein binding pre-mRNA binding phospholipase binding primary miRNA binding protein-RNA sequence-specific adaptor activity sequence-specific mRNA binding

Process(es)

regulation of alternative mRNA splicing, via spliceosome RNA processing mRNA export from nucleus primary miRNA processing regulation of mRNA splicing, via spliceosome

Performance Guarantee

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SRSF3 Monoclonal Antibody (1H4G7)

SRSF3 Antibody (33-9400) in WB

Product Details

33-9400

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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