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Invitrogen
MUC4 Monoclonal Antibody (1G8)
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FIGURE: 1 / 9
MUC4 Antibody (35-4900) in ICC/IF
Immunofluorescence analysis of MUC4/Mucin 4 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUC4/Mucin 4 (1G8) Mouse Monoclonal Antibody (Product # 35-4900) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
35-4900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1G8
Immunogen
Rat ASGP-2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978). These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978). Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ascites sialoglycoprotein; ASGP; ASGP1; Heterodimeric complex composed of a mucin subunit, ASGP-1, which is predominantly O-glycosylated, and a cysteine-rich transmembrane subunit, ASGP-2, which is predominantly N-glycosylated; large membrane-bound mucin; MUC-4; MUC4; MUC4; ascites sialoglycoprotein-2; ASGP-2; mucin 4, ASGP; mucin 4, tracheobronchial; Mucin4; pancreatic adenocarcinoma mucin; pre-sialomucin complex; pSMC; repeat sequence B; repeat sequence C; repeat sequence D; repeat sequence E; sialomucin ascites sialoglycoprotein-1; sialomucin complex; testis mucin; tracheobronchial mucin; unnamed protein product
Gene Aliases: 4933405I11Rik; ASGP; ASGP-1; HSA276359; MUC-4; Psmc
UniProt ID: (Human) Q99102, (Mouse) Q8JZM8
Entrez Gene ID: (Human) 4585, (Mouse) 140474, (Rat) 303887
cell-matrix adhesion
maintenance of gastrointestinal epithelium
negative regulation of cell-matrix adhesion
hematopoietic progenitor cell differentiation
negative regulation of T cell mediated cytotoxicity directed against tumor cell target
regulation of receptor activity
negative regulation of cell-cell adhesion
negative regulation of apoptotic process
lactation
response to xenobiotic stimulus
response to hormone
mammary gland development
post-embryonic camera-type eye development
response to estradiol
response to progesterone
cellular response to insulin stimulus
response to vitamin A
response to cytokine
estrous cycle
digestive tract development
cellular response to transforming growth factor beta stimulus
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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