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Invitrogen
Connexin 43 Monoclonal Antibody (3D8A5)
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FIGURE: 1 / 55
Connexin 43 Antibody (35-5000) in IHC
Double immunofluorescent staining of mouse heart atrium using mouse anti-Connexin 43 monoclonal antibody (Product # 35-5000) (red) and Rabbit anti-Connexin 40 (C-term, Product # 36-4900) (green). Cx43 immunolocalizes to the intercalated discs throughout cardiac tissue, while Cx40 immunolabeling is localized to intercalated discs restricted to the conduction system. Image courtesy of James I. Nagy, PhD, Univ... View More
Product Details
35-5000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3D8A5
Immunogen
Synthetic peptide derived from rat Connexin 43.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; ODD; ODOD; SDTY3; Syndactyly type III
Gene Aliases: AU042049; AW546267; Cnx43; connexin43; Cx43; Cx43alpha1; Cxn-43; Gja-1; Gja1; Npm1
UniProt ID: (Mouse) P23242, (Rat) P08050
Entrez Gene ID: (Mouse) 14609, (Rat) 24392
signal transducer activity
receptor binding
gap junction channel activity
protein binding
ion transmembrane transporter activity
SH3 domain binding
protein domain specific binding
transmembrane transporter activity
PDZ domain binding
beta-tubulin binding
connexin binding
scaffold protein binding
gap junction channel activity involved in cell communication by electrical coupling
gap junction
osteoblast differentiation
in utero embryonic development
neuron migration
negative regulation of endothelial cell proliferation
heart looping
regulation of heart rate
epithelial cell maturation
lens development in camera-type eye
chronic inflammatory response
positive regulation of glomerular filtration
atrial ventricular junction remodeling
epicardial cell to mesenchymal cell transition
apoptotic process
cell communication
signal transduction
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
heart development
adult heart development
regulation of heart contraction
negative regulation of cell proliferation
response to pH
vascular transport
positive regulation of gene expression
negative regulation of gene expression
cell communication by chemical coupling
cell communication by electrical coupling
positive regulation of cell communication by chemical coupling
ATP transport
regulation of bone mineralization
positive regulation of insulin secretion
ion transmembrane transport
embryonic heart tube development
embryonic digit morphogenesis
positive regulation of I-kappaB kinase/NF-kappaB signaling
skeletal muscle tissue regeneration
cell-cell junction organization
positive regulation of osteoblast differentiation
positive regulation of protein catabolic process
positive regulation of striated muscle tissue development
positive regulation of vasoconstriction
positive regulation of vasodilation
regulation of bone remodeling
blood vessel morphogenesis
neuron projection morphogenesis
protein oligomerization
regulation of calcium ion transport
transmembrane transport
negative regulation of cardiac muscle cell proliferation
milk ejection
regulation of ventricular cardiac muscle cell membrane repolarization
regulation of blood vessel remodeling
regulation of atrial cardiac muscle cell membrane depolarization
regulation of ventricular cardiac muscle cell membrane depolarization
negative regulation of wound healing
cardiac conduction
establishment of epithelial cell polarity
negative regulation of DNA biosynthetic process
regulation of bicellular tight junction assembly
positive regulation of behavioral fear response
endothelium development
response to glucose
gap junction assembly
response to fluid shear stress
response to peptide hormone
cellular response to mechanical stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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