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Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6)
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FIGURE: 1 / 8
Phospho-Tau (Ser396) Antibody (35-5300) in WB
Western blot was performed using Anti-Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6) (Product # 35-5300) and a 50kDa band corresponding to Phospho-Tau (Ser396) was observed across cell line tested and increased upon Retinoic acid and the successive Okadaic acid treatments. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with Retinoic acid (10 uM for 8 days) (Lane 2), SH-SY5Y diff... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20240507?time=20240507 "Phospho-Tau (Ser396) Antibody (35-5300) in IHC")
Product Details
35-5300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Dog,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
PHF13.6
Immunogen
Purified human PHF-Tau preparation
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This monoclonal antibody recognizes phosphorylation-dependent epitopes of ser-396 on PHF-tau as well as fetal tau, but does not recognize autopsy-derived normal adult tau. Phosphoamino ser-396 is one of the two major immunodominant residues of PHF-tau. PHF 13.6 does not recognize unphosphorylated peptide 390-408 or the same peptide phosphorylated at ser-400, thr-403, or ser-404. PHF 13.6 specifically recognizes the ser-396 phosphorylated peptide and all diphosphorylated peptides containing phosphorylated ser-396. Reactivity was confirmed with human PHF-Tau.
Target Information
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MAPT; MAPT_BigTau; MGC138549; microtubule-associated protein tau; microtubule-associated protein tau 0N4R; microtubule-associated protein tau 1N4R; microtubule-associated protein tau 2N4R; microtubule-associated protein tau BigTau; microtubules; neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; tau microtubule binding protein; Tau microtubule-associated protein; Tau-4; Tau-derived paired helical filament hexapeptide; Tau5; unnamed protein product
Gene Aliases: DDPAC; FTD1; FTDP-17; MAPT_0N4R; MAPT_1N4R; MAPT_2N4R; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PHF-tau; PPND; PPP1R103; pTau; RNPTAU; TAU; tau-40; Tau-PHF6
UniProt ID: (Human) P10636, (Mouse) P10637
Entrez Gene ID: (Human) 4137, (Mouse) 17762, (Rat) 29477
DNA binding
AT DNA binding
double-stranded DNA binding
single-stranded DNA binding
RNA binding
actin binding
enzyme inhibitor activity
protein binding
microtubule binding
tubulin binding
SH3 domain binding
enzyme binding
protein kinase binding
protein binding, bridging
apolipoprotein binding
dynactin binding
phosphatidylinositol binding
identical protein binding
sequence-specific DNA binding
chaperone binding
protein phosphatase 2A binding
Hsp90 protein binding
lipoprotein particle binding
histone-dependent DNA binding
microtubule lateral binding
microtubule stabilizing activity
phosphatidylinositol bisphosphate binding
heat shock protein binding
macromolecular complex binding
microtubule cytoskeleton organization
microglial cell activation
cellular response to DNA damage stimulus
cell-cell signaling
synapse assembly
learning or memory
memory
response to lead ion
regulation of autophagy
negative regulation of gene expression
negative regulation of mitochondrial membrane potential
rRNA metabolic process
axonal transport of mitochondrion
central nervous system neuron development
regulation of microtubule polymerization or depolymerization
regulation of microtubule polymerization
positive regulation of microtubule polymerization
cytoplasmic microtubule organization
neuron projection development
positive regulation of superoxide anion generation
regulation of chromosome organization
stress granule assembly
cellular response to heat
cellular response to reactive oxygen species
positive regulation of axon extension
microtubule polymerization
astrocyte activation
regulation of synaptic plasticity
intracellular distribution of mitochondria
generation of neurons
synapse organization
regulation of calcium-mediated signaling
protein polymerization
protein homooligomerization
regulation of microtubule-based movement
axon development
regulation of microtubule cytoskeleton organization
plus-end-directed organelle transport along microtubule
regulation of mitochondrial fission
negative regulation of mitochondrial fission
fibril organization
axonal transport
regulation of cellular response to heat
regulation of long term synaptic depression
positive regulation of protein localization to synapse
neurofibrillary tangle assembly
negative regulation of establishment of protein localization to mitochondrion
positive regulation of cellular protein localization
amyloid fibril formation
cellular response to nerve growth factor stimulus
cellular response to brain-derived neurotrophic factor stimulus
neuron migration
axonogenesis
response to nutrient
adult walking behavior
axo-dendritic transport
intrinsic apoptotic signaling pathway in response to oxidative stress
positive regulation of mitochondrial membrane potential
positive regulation of neuron projection development
negative regulation of intracellular transport
mitochondrion transport along microtubule
axon extension
mRNA transport
response to hyperoxia
positive regulation of long term synaptic depression
negative regulation of tubulin deacetylation
positive regulation of mesenchymal stem cell migration
microtubule-based process
female pregnancy
response to gamma radiation
neuron differentiation
response to ethanol
response to arsenic-containing substance
cellular response to calcium ion
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