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Invitrogen
Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody
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FIGURE: 1 / 13
Phospho-p38 MAPK (Thr180, Tyr182) Antibody (36-8500) in ICC/IF
Immunofluorescence analysis of Phospho-p38 MAPK alpha (Thr180, Tyr182) was performed using 70% confluent log phase HeLa cells treated with UV for 40 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-p38 MAPK alpha (Thr180, Tyr182) Polyclonal An... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
36-8500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to a region surrounding the phosphorylated Thr180/Tyr182 residues of p38 MAP kinase.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primates and zebrafish based on 100% sequence homology.
Target Information
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CSAID-binding protein; cytokine suppressive anti-inflammatory drug binding protein; EC 2.7.11.24; encoded by transcript variant 4; p38 MAP kinase; encoded by transcript variant 5; p38 MAP kinase; ERK1; ERT2; MAP 14; MAP kinase 1; MAP kinase 14; MAP kinase Mxi2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAPK1 antibody; MAPK14; MAX-interacting protein 2; mitogen-activated protein kinase p38 alpha; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; RP1-179N16.5; SAPK2a; CSAIDS binding protein 1; stress-activated protein kinase 2A; unnamed protein product
Gene Aliases: CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38; p38ALPHA; PRKM14; PRKM15; RK; SAPK2A
UniProt ID: (Human) Q16539
Entrez Gene ID: (Human) 1432
MAPK cascade
negative regulation of inflammatory response to antigenic stimulus
regulation of transcription from RNA polymerase II promoter
chemotaxis
signal transduction
cell surface receptor signaling pathway
positive regulation of gene expression
positive regulation of myotube differentiation
platelet activation
osteoclast differentiation
stress-activated protein kinase signaling cascade
positive regulation of cyclase activity
positive regulation of interleukin-12 production
negative regulation of hippo signaling
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
p38MAPK cascade
signal transduction in response to DNA damage
positive regulation of blood vessel endothelial cell migration
positive regulation of erythrocyte differentiation
positive regulation of myoblast differentiation
vascular endothelial growth factor receptor signaling pathway
positive regulation of muscle cell differentiation
stress-activated MAPK cascade
3'-UTR-mediated mRNA stabilization
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to ionizing radiation
cellular response to UV-B
cellular senescence
stress-induced premature senescence
cellular response to virus
regulation of cytokine production involved in inflammatory response
positive regulation of myoblast fusion
positive regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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