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Invitrogen

Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody

Advanced Verification
10 Published Figures
24 References
View all (86) ERK1/2 antibodies

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Product Details

36-8800

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 16 publications 16 publications

Immunohistochemistry (IHC)

1:500
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:250
View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Plant, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to the region surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1+2 (p44/42 MAP kinase).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533283

Product Specific Information

This antibody is predicted to react with Xenopus and zebrafish based on 100% sequence homology.

36-8800 is specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ERK-2; erk1 erk2; Erk1; cancer susceptibility protein; ERK1; MAP kinase; mitogen-activated protein kinase; ERK1b; ERK1c; Erk2; cancer susceptibility protein; ERT1; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAPK 1; MAPK 2; MAPK 3; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; pp42/MAP kinase; protein serine/threonine kinase ERK1b; protein tyrosine kinase ERK2; unnamed protein product

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Gene Aliases: 9030612K14Rik; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK2; MNK1; Mtap2k; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708

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Entrez Gene ID: (Human) 5594, (Human) 5595, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689

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Function(s)
phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding double-stranded DNA binding protein kinase binding mitogen-activated protein kinase kinase kinase binding scaffold protein binding
Process(es)
autophagosome assembly MAPK cascade negative regulation of T cell mediated immune response to tumor cell protein phosphorylation cellular response to DNA damage stimulus signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway insulin receptor signaling pathway response to toxic substance organ morphogenesis negative regulation of autophagy positive regulation of autophagy positive regulation of macrophage chemotaxis neural crest cell development Schwann cell development phosphorylation peptidyl-serine phosphorylation sensory perception of pain regulation of ossification BMP signaling pathway thyroid gland development positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway cellular response to nutrient levels positive regulation of telomere maintenance negative regulation of proteasomal ubiquitin-dependent protein catabolic process response to lipopolysaccharide regulation of stress-activated MAPK cascade cellular response to amino acid starvation intracellular signal transduction peptidyl-tyrosine autophosphorylation ERBB2-ERBB3 signaling pathway TORC1 signaling outer ear morphogenesis myelination signal transduction in response to DNA damage response to exogenous dsRNA positive regulation of translation positive regulation of transcription from RNA polymerase II promoter decidualization insulin-like growth factor receptor signaling pathway thymus development modulation of synaptic transmission protein stabilization negative regulation of T cell activation cartilage development stress-activated MAPK cascade regulation of intracellular pH regulation of cytoskeleton organization Bergmann glial cell differentiation face development lung morphogenesis trachea formation cardiac neural crest cell development involved in heart development interleukin-34-mediated signaling pathway protein-containing complex assembly ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus cellular response to tumor necrosis factor caveolin-mediated endocytosis regulation of Golgi inheritance negative regulation of cholesterol efflux cellular response to toxic substance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response negative regulation of TORC1 signaling positive regulation of xenophagy regulation of early endosome to late endosome transport arachidonic acid metabolic process regulation of transcription from RNA polymerase II promoter apoptotic process chemotaxis chemical synaptic transmission learning or memory positive regulation of peptidyl-threonine phosphorylation response to nicotine ERBB signaling pathway positive regulation of skeletal muscle tissue regeneration positive regulation of cholesterol biosynthetic process positive regulation of smoothened signaling pathway chemokine-mediated signaling pathway heart development positive regulation of cell proliferation diadenosine tetraphosphate biosynthetic process cytosine metabolic process positive regulation of cell migration response to estradiol response to testosterone mammary gland epithelial cell proliferation cellular response to platelet-derived growth factor stimulus response to cocaine positive regulation of protein import into nucleus response to hydrogen peroxide response to estrogen estrous cycle negative regulation of cell differentiation positive regulation of transcription, DNA-templated T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of cardiac muscle cell proliferation long-term synaptic potentiation labyrinthine layer blood vessel development cellular response to methionine cellular response to cAMP cellular response to epidermal growth factor stimulus cellular response to peptide hormone stimulus cellular response to prostaglandin E stimulus cellular response to lipid response to alcohol cellular response to insulin-like growth factor stimulus placenta development
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