Invitrogen

BTRC Monoclonal Antibody (1B1D2)

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29 Published Figures

36 References

View all (11) BTRC antibodies

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Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

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Additional Information:

BTRC Antibody in Immunocytochemistry (ICC/IF)

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BTRC Antibody (37-3400) in ICC/IF

Immunofluorescent analysis of BTRC was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BTRC (1B1D2) Mouse Monoclonal Antibody (Product # 37-3400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

BTRC Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

BTRC Antibody in Western Blot, Immunoprecipitation (WB, IP)

BTRC Antibody in Western Blot, ChIP Assay (WB, ChIP)

BTRC Antibody in Western Blot, Flow Cytometry (WB, Flow)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

BTRC Antibody — Figure 5 NNK enhances hnRNP-U/betaTrCP translocation to the cytoplasm and induces DNMT1 accumulation in the nucleus, but this effect is attenuated by AKT inhibition. ( A ) Immunofluorescence confocal microscopy to localize DNMT1 (green), betaTrCP (red), and nucleus (blue; DAPI). The merged figures showed colocalization of DNMT1 with betaTrCP protein in A549 cells treated with DMSO control. Mutually exclusive localization of DNMT1 and betaTrCP was observed in cells treated with NNK. Original magnification, x630. Scale bar: 20 mum. ( B ) Cell fractionation assay to analyze distribution of nuclear and cytoplasmic proteins, using anti-DNMT1, anti-betaTrCP, anti-hnRNP-U, and anti-GSK3beta Ser9 antibodies. Anti-GAPDH and anti-lamin A/C antibodies were used as cytoplasmic and nuclear protein markers, respectively. The distribution of betaTrCP and hnRNP-U proteins was contrary to that of DNMT1 protein. ( C ) DNMT1 and betaTrCP interaction was interrupted by NNK treatment. In contrast, betaTrCP and hnRNP interaction was increased by NNK treatment. ( D ) Cell fractionation assay was performed in A549 cells with or without AKT siRNA. Inhibition of AKT induced both hnRNP-U and betaTrCP proteins predominantly located in the nucleus, where DNMT1 protein level decreased. ( E ) IP assay using anti-AKT antibody showed that hnRNP formed a complex with AKT, and the interaction was attenuated by LY294002. ( F ) An increase of hnRNP-U phosphorylation was mediated by exogenous AKT, which increased {TM}

Product Details

37-3400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 8 publications 8 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Proximity Ligation Assay (PLA) (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1B1D2

Immunogen

Recombinant human beta-TrCP fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Ambient (domestic); Wet ice (international)

AB_2533313

Target Information

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta-transducin repeat containing protein; beta-transducin repeats-containing protein; beta-TrCP1; E3RSIkappaB; epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; F-box and WD-repeat protein 1B; pIkappaBalpha-E3 receptor subunit; unnamed protein product

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Gene Aliases: BETA-TRCP; beta-TrCP1; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1

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UniProt ID: (Human) Q9Y297

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Entrez Gene ID: (Human) 8945, (Rat) 361765

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Function(s)

protein binding beta-catenin binding protein phosphorylated amino acid binding protein dimerization activity ubiquitin protein ligase activity protein binding, bridging involved in substrate recognition for ubiquitination ubiquitin ligase activator activity ubiquitin-protein transferase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination protein dephosphorylation ubiquitin-dependent protein catabolic process signal transduction regulation of cell cycle process protein processing protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process NIK/NF-kappaB signaling regulation of circadian rhythm positive regulation of circadian rhythm proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of smoothened signaling pathway positive regulation of transcription, DNA-templated negative regulation of T cell receptor signaling pathway regulation of canonical Wnt signaling pathway protein K48-linked ubiquitination protein catabolic process mammary gland epithelial cell proliferation regulation of I-kappaB kinase/NF-kappaB signaling regulation of cell cycle branching involved in mammary gland duct morphogenesis regulation of proteasomal protein catabolic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BTRC Monoclonal Antibody (1B1D2)

BTRC Antibody (37-3400) in ICC/IF

Product Details

37-3400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Proximity Ligation Assay (PLA) (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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