Search
Invitrogen
EphA2 Monoclonal Antibody (1C11A12)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 13
EphA2 Antibody (37-4400) in ICC/IF
Immunofluorescent analysis of EphA2 was performed using 70% confluent log phase A549 cells treated with UV-302 nm for 60 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EphA2 (1C11A12) Mouse Monoclonal Antibody (Product # 37-4400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Ale... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
37-4400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1C11A12
Immunogen
Synthetic peptide derived from the C-terminal region of the human EphA2 Receptor protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA2 is overexpressed in many types of human cancers. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ECK; ephrin receptor EphA2; epithelial cell kinase; epithelial cell receptor protein tyrosine kinase; Sek-2; tyrosine-protein kinase receptor ECK; tyrosine-protein kinase receptor MPK-5; tyrosine-protein kinase receptor SEK-2; unnamed protein product
Gene Aliases: ARCC2; CTPA; CTPP1; CTRCT6; ECK; Myk2; Sek-2; Sek2
UniProt ID: (Human) P29317, (Mouse) Q03145
Entrez Gene ID: (Human) 1969, (Mouse) 13836, (Rat) 366492
skeletal system development
angiogenesis
blood vessel development
vasculogenesis
osteoblast differentiation
negative regulation of cytokine production
blood vessel endothelial cell proliferation involved in sprouting angiogenesis
response to stress
inflammatory response
transmembrane receptor protein tyrosine kinase signaling pathway
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of lamellipodium assembly
notochord formation
cell migration
negative regulation of angiogenesis
neural tube development
neuron differentiation
keratinocyte differentiation
osteoclast differentiation
regulation of cell migration
positive regulation of cell migration
epithelial cell differentiation
negative regulation of chemokine production
mammary gland epithelial cell proliferation
regulation of cell adhesion mediated by integrin
negative regulation of cell adhesion mediated by integrin
post-anal tail morphogenesis
regulation of blood vessel endothelial cell migration
regulation of angiogenesis
cAMP metabolic process
bone remodeling
ephrin receptor signaling pathway
axial mesoderm formation
animal organ development
blood vessel morphogenesis
notochord morphogenesis
cell motility
regulation of developmental process
defense response to Gram-positive bacterium
notochord cell development
cell chemotaxis
branching involved in mammary gland duct morphogenesis
lens fiber cell morphogenesis
regulation of ERK1 and ERK2 cascade
response to growth factor
protein localization to plasma membrane
activation of GTPase activity
negative regulation of lymphangiogenesis
positive regulation of protein localization to plasma membrane
positive regulation of bicellular tight junction assembly
pericyte cell differentiation
regulation of cell adhesion
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support