Blocker™ BLOTTO in TBS

Catalog number: 37530

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Thermo Scientific Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.

Features of Blocker BLOTTO Blocking Buffer:

Popular—nonfat milk has been used for many years in a variety of protein methods, although it is not recommended for avidin-based techniques because it contains some endogenous biotin
Convenient—supplied as a ready-to-use 1X TBS solution; can be diluted as needed
Easy to use—formulated with anti-foaming agent and thimerosal-free preservative
Flexible—may be used for multiple applications, including as a diluent for antibody

The purpose of the blocking step in an assay is to improve assay sensitivity by reducing background interference. However, unforeseen cross-reaction of detection reagents with blocking buffers is itself a cause of high background and low signal-to-noise ratios in assay systems. Because individual blocking buffers are not compatible with every system, a variety of blockers in both Tris-buffered saline (TBS) and phosphate-buffered saline (PBS) are available. The best blocking buffer for a specific experiment will bind to all potential sites of nonspecific interaction, eliminating background without altering or obscuring the epitope for antibody binding.

To optimize the blocking step for a particular immunoassay, empirical testing is essential. Using inadequate amounts of blocker will result in excessive background. Using an excessive blocker concentration can mask antibody-antigen interactions or inhibit the marker enzyme. For best results when developing a new immunoassay, test several different blocking agents for the highest signal-to-noise ratio in the assay. There is no single blocking agent that is ideal for every occasion because many factors can influence nonspecific binding, including various protein interactions unique to a specific assay system.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Product Size: 1 L
Reagent Type: Blocking Reagent
Validated Application: ELISA, Western Blot

Contents & storage

Store at 4°C.

Documents

Manuals & protocols